Team:Paris/Transduction overview

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[[Team:Paris/Transduction_overview_strategy#bottom |C. Our strategy]] and [[Team:Paris/Transduction_overview_construction#bottom |D. Construction]] part explains our aim in this OMV production system.  
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[[Team:Paris/Transduction_overview_strategy#bottom | Our strategy]] and [[Team:Paris/Transduction_overview_construction#bottom | Construction]] part explains our aim in this OMV production system.  

Revision as of 14:31, 4 September 2009

iGEM > Paris > Reception > Overview

Introduction

This part of the project was focus on two points:

  • The fusion between the OMVs and the targeted bacteria.
  • The activation of the transcription of a genetic construction after fusionning the OMVs with the outer membrane of the receiving bacterium.


The sinequanone conditions : we tried to achieve this aim without sacrifying important proprieties of our message : specific, repeatable , multidirectional.


It seems that we have two possible ways : the ABC transporters or the two component systems .


ABC transporters and two component systems are natural transport systems (export or import) of nutriments or toxines, and even information, the mecanism is mostly unknown but the DNA-containing OMVs could be a useful mean of information transport. Here for information about ABC transporter and Two Component System.


Our strategy and Construction part explains our aim in this OMV production system.


References

  1. ^ D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. Lopilato JE & Beckwith JR. 1984 - 6327616
  2. ^ Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface. Härle C & Braun V. 1995 - 7729419
  3. ^ A comparative analysis of ABC transporters in complete microbial genomes. Tomii K & Kanehisa M. 1998- 9799792
  4. ^ The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.De Wulf P & Lin EC. 1999 - 10542180
  5. ^ Two-component signal transduction. Stock AM & Goudreau PN. 2000- 10966457
  6. ^ Vesicle-mediated transfer of virulence genes from Escherichia coli O157:H7 to other enteric bacteria. Yaron S & Matthews KR. 2000- 11010892
  7. ^ Periplasmic binding proteins: a versatile superfamily for protein engineering. Dwyer MA & Hellinga HW. 2004- 11010892
  8. ^ Gene regulation by transmembrane signaling. Braun V & Sauter A. 2006- 16718597
  9. ^ Signal transduction: networks and integrated circuits in bacterial cognition. Baker MD & Stock JB 2007- 18054766
  10. ^ Systems biology of bacterial chemotaxis. Baker MD & Stock JB. 2007- 16529985
  11. ^ Control of the transcription of a short gene encoding a cyclic peptide in Streptococcus thermophilus: a new quorum-sensing system? Ibrahim M & Monnet V. 2007- 17921293
  12. ^ SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli. Thie H & Hust M. 2008- 18504019
  13. ^ Signal transduction and adaptive regulation through bacterial two-component systems: the Escherichia coli AtoSC paradigm. Kyriakidis DA & Tiligada E. 2009- 19198978
  14. ^ comparative analysis of ABC transporter. Tomii & Kanehisa 2009- pdf-link