Team:Paris/Transduction overview

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Overview

• Introduction
• A. Fusion
  • A.1 jun/fos
  • A.2 G3P
  • A.3 Snares
• B. Transduction
  • B.1 ABC transporters
  • B.2 Two Component System
• C. Our strategy
• D. Construction

Introduction

This part of the project was focus on two points:

fusion between the vesicle and the targeted bacteria.

- to enable gene transcription after fusionning OMVs with the outer membrane of the receiving bacterium.

We also hope that we could achieve this aim without sacrifying important proprieties of our message : specific, repeatable , multidirectional. It seems that we have two possible ways : the ABC transporters or the two component systems . ABC transporters and two component systems are natural transport system (export or import) of information, nutriments or toxines.

There is another possibility but the mecanism is mostly unknown : the DNA-containing OMVs which could be a useful mean of information transport.

References

1. 1 D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. Lopilato JE & Beckwith JR. 1984 - 6327616
2. 2 Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface. Härle C & Braun V. 1995 - 7729419
3. 3 A comparative analysis of ABC transporters in complete microbial genomes. Tomii K & Kanehisa M. 1998- 9799792
4. 4 The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.De Wulf P & Lin EC. 1999 - 10542180
5. 5 Two-component signal transduction. Stock AM & Goudreau PN. 2000- 10966457
6. 6 Vesicle-mediated transfer of virulence genes from Escherichia coli O157:H7 to other enteric bacteria. Yaron S & Matthews KR. 2000- 11010892
7. 7 Periplasmic binding proteins: a versatile superfamily for protein engineering. Dwyer MA & Hellinga HW. 2004- 11010892
8. 8 Gene regulation by transmembrane signaling. Braun V & Sauter A. 2006- 16718597
9. 9 Signal transduction: networks and integrated circuits in bacterial cognition. Baker MD & Stock JB 2007- 18054766
10. 10 Systems biology of bacterial chemotaxis. Baker MD & Stock JB. 2007- 16529985
11. 11 Control of the transcription of a short gene encoding a cyclic peptide in Streptococcus thermophilus: a new quorum-sensing system? Ibrahim M & Monnet V. 2007- 17921293
12. 12 SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli. Thie H & Hust M. 2008- 18504019
13. 13 Signal transduction and adaptive regulation through bacterial two-component systems: the Escherichia coli AtoSC paradigm. Kyriakidis DA & Tiligada E. 2009- 19198978
14. 14 comparative analysis of ABC transporter. Tomii & Kanehisa 2009- pdf-link