Team:Paris/Transduction overview

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Introduction

This part of the project was focus on two points:


The sinequanone conditions : we tried to achieve this aim without sacrifying important proprieties of our message : specific, repeatable , multidirectional.


It seems that we have two possible ways : the ABC transporters or the two component systems .


LE BIG SCHEMA : style 2 bactouze , l'émettrice envoie vesicule , avec fec in the membrane, et prot dedans , et sur le schéma les deux path ways , style fec le message est capt dans la outermembrane et transduit (abc transporteer trick), pour l'autre la prot rentre dans le périplasme est capté puis un récepteur mb interbne chope la molécule choppé et transduit le signal


ABC transporters and two component systems are natural transport systems (export or import) of nutriments or toxines, and even information but the mecanism is mostly unknown so the DNA-containing OMVs would have been a useful mean of information transport if it would not be so unknown. Here for information about ABC transporter and Two Component System.


Our strategy and Construction part explains our aim in this OMV production system.


References

  1. ^ D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. Lopilato JE & Beckwith JR. 1984 - 6327616
  2. ^ Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface. Härle C & Braun V. 1995 - 7729419
  3. ^ A comparative analysis of ABC transporters in complete microbial genomes. Tomii K & Kanehisa M. 1998- 9799792
  4. ^ The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.De Wulf P & Lin EC. 1999 - 10542180
  5. ^ Two-component signal transduction. Stock AM & Goudreau PN. 2000- 10966457
  6. ^ Vesicle-mediated transfer of virulence genes from Escherichia coli O157:H7 to other enteric bacteria. Yaron S & Matthews KR. 2000- 11010892
  7. ^ Periplasmic binding proteins: a versatile superfamily for protein engineering. Dwyer MA & Hellinga HW. 2004- 11010892
  8. ^ Gene regulation by transmembrane signaling. Braun V & Sauter A. 2006- 16718597
  9. ^ Signal transduction: networks and integrated circuits in bacterial cognition. Baker MD & Stock JB 2007- 18054766
  10. ^ Systems biology of bacterial chemotaxis. Baker MD & Stock JB. 2007- 16529985
  11. ^ Control of the transcription of a short gene encoding a cyclic peptide in Streptococcus thermophilus: a new quorum-sensing system? Ibrahim M & Monnet V. 2007- 17921293
  12. ^ SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli. Thie H & Hust M. 2008- 18504019
  13. ^ Signal transduction and adaptive regulation through bacterial two-component systems: the Escherichia coli AtoSC paradigm. Kyriakidis DA & Tiligada E. 2009- 19198978
  14. ^ comparative analysis of ABC transporter. Tomii & Kanehisa 2009- pdf-link