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Team:Queens/Notebook
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<p>-Finalized to-do list | <p>-Finalized to-do list | ||
<p>-Mini-prepped the following: | <p>-Mini-prepped the following: | ||
| - | <p> -Linker K157013 <i>Plate 3, Well 3G, plasmid Bba_K157000(A)</i> | + | <p> -Linker K157013 <i>Plate 3, Well 3G, plasmid Bba_K157000(A)</i> |
| - | <p> -TEV Protease | + | <p> -TEV Protease |
| - | <p> | + | <p> -I712078 (C-Terminus) <i>Plate 2, Well 14M, plasmid J70003 (A)</i> |
| - | <p> | + | <p> -I712077 (N-Terminus) <i>Plate 2, Well 14K, plasmid J70003 (A)</i> |
| - | <p> -pLux | + | <p> -pLux |
| - | <p> | + | <p> -R0062 (not leaky) <i>Plate 1, Well 6O, plasmid pSB1A2 (A)</i> |
| - | <p> | + | <p> -R1062 (median strength in the absence of luxR/HSL) <i>Plate 1, Well 8G, plasmid pSB1A2 (A)</i> |
| - | <p> -HO-pcyA | + | <p> -HO-pcyA |
| - | <p> | + | <p> -K098010 <i>Plate 3, Well 11N, plasmid pSB3k3 (K)</i> |
| - | <p> -Terminator | + | <p> -Terminator |
| - | <p> | + | <p> -B0015 <i>Plate 1, Well 23L, plasmid pSB1AK3 (AK)</i> |
| - | <p> -RFP | + | <p> -RFP |
| - | <p> | + | <p> -E1010 <i>Plate 1, Well 18F, plasmid pSB2K3 (K)</i> |
Revision as of 12:46, 16 July 2009
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Laboratory One: Harry, Bogdan, James, Bryant Laboratory Two: Kate, Mike Tech Support and Laboratory Three: Parthiv, Chris, Chris |
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Laboratory One: Harry, Bogdan, James, Bryant 16/06/2009
-Lab Meeting -Finished the sequence for the surface expression construct -Finalized to-do list -Mini-prepped the following: -Linker K157013 Plate 3, Well 3G, plasmid Bba_K157000(A) -TEV Protease -I712078 (C-Terminus) Plate 2, Well 14M, plasmid J70003 (A) -I712077 (N-Terminus) Plate 2, Well 14K, plasmid J70003 (A) -pLux -R0062 (not leaky) Plate 1, Well 6O, plasmid pSB1A2 (A) -R1062 (median strength in the absence of luxR/HSL) Plate 1, Well 8G, plasmid pSB1A2 (A) -HO-pcyA -K098010 Plate 3, Well 11N, plasmid pSB3k3 (K) -Terminator -B0015 Plate 1, Well 23L, plasmid pSB1AK3 (AK) -RFP -E1010 Plate 1, Well 18F, plasmid pSB2K3 (K) |
