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Team:Queens/Notebook
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| + | <table style="background-color:#922334; position:relative; overflow:auto; left:200px; top:-215px; width:750px"> | ||
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| + | <td align="left"><p style="font-size:175%;font-family:corbel;color:#172C4E;font-weight:bold"><i>Menu<i> | ||
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| + | <p style="font-size:150%;font-family:corbel;color:#172C4E;font-weight:bold"><a href="#Lab1">Laboratory One: Harry, Bogdan, James, Bryant</a></p> | ||
| + | <p style="font-size:150%;font-family:corbel;color:#172C4E;font-weight:bold"><a href="#Lab2">Laboratory Two: Kate, Mike</a></p> | ||
| + | <p style="font-size:150%;font-family:corbel;color:#172C4E;font-weight:bold"><a href="#Tech">Tech Support and Laboratory Three: Parthiv, Chris, Chris</a></p> | ||
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| + | <p style="font-size:150%;font-family:corbel;color:#172C4E;font-weight:bold"><a name="Lab1">Laboratory One: Harry, Bogdan, James, Bryant</a></p> | ||
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| + | <p style="font-size:120%;font-family:palatino linotype;color:#ECB528">16/06/2009</p> | ||
| + | <p style="font-size:120%;font-family:palatino linotype;color:#ECB528"> | ||
| + | -Lab Meeting | ||
| + | -Finished the sequence for the surface expression construct | ||
| + | -Finalized to-do list | ||
| + | -Mini-prepped the following: | ||
| + | -Linker K157013 <i>Plate 3, Well 3G, plasmid Bba_K157000(A)</i> | ||
| + | -TEV Protease | ||
| + | -I712078 (C-Terminus) <i>Plate 2, Well 14M, plasmid J70003 (A)</i> | ||
| + | -I712077 (N-Terminus) <i>Plate 2, Well 14K, plasmid J70003 (A)</i> | ||
| + | -pLux | ||
| + | -R0062 (not leaky) <i>Plate 1, Well 6O, plasmid pSB1A2 (A)</i> | ||
| + | -R1062 (median strength in the absence of luxR/HSL) <i>Plate 1, Well 8G, plasmid pSB1A2 (A)</i> | ||
| + | -HO-pcyA | ||
| + | -K098010 <i>Plate 3, Well 11N, plasmid pSB3k3 (K)</i> | ||
| + | -Terminator | ||
| + | -B0015 <i>Plate 1, Well 23L, plasmid pSB1AK3 (AK)</i> | ||
| + | -RFP | ||
| + | -E1010 <i>Plate 1, Well 18F, plasmid pSB2K3 (K)</i> | ||
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Revision as of 12:36, 16 July 2009
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| Laboratory One: Harry, Bogdan, James, Bryant
16/06/2009 -Lab Meeting -Finished the sequence for the surface expression construct -Finalized to-do list -Mini-prepped the following: -Linker K157013 Plate 3, Well 3G, plasmid Bba_K157000(A) -TEV Protease -I712078 (C-Terminus) Plate 2, Well 14M, plasmid J70003 (A) -I712077 (N-Terminus) Plate 2, Well 14K, plasmid J70003 (A) -pLux -R0062 (not leaky) Plate 1, Well 6O, plasmid pSB1A2 (A) -R1062 (median strength in the absence of luxR/HSL) Plate 1, Well 8G, plasmid pSB1A2 (A) -HO-pcyA -K098010 Plate 3, Well 11N, plasmid pSB3k3 (K) -Terminator -B0015 Plate 1, Well 23L, plasmid pSB1AK3 (AK) -RFP -E1010 Plate 1, Well 18F, plasmid pSB2K3 (K) |
