Team:Queens/Notebook
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<a name="Lab1"><p style="font-size:150%;font-family:corbel;color:#172C4E;font-weight:bold">Laboratory One: Harry, Bogdan, James, Bryant</p></a> | <a name="Lab1"><p style="font-size:150%;font-family:corbel;color:#172C4E;font-weight:bold">Laboratory One: Harry, Bogdan, James, Bryant</p></a> | ||
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<p style="font-size:120%;font-family:palatino linotype;color:#ECB528">16/06/2009</p> | <p style="font-size:120%;font-family:palatino linotype;color:#ECB528">16/06/2009</p> | ||
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- | + | -Lab Meeting | |
- | + | -Finished the sequence for the surface expression construct | |
- | + | -Finalized to-do list | |
- | + | -Mini-prepped the following: | |
- | + | -Linker K157013 <i>Plate 3, Well 3G, plasmid Bba_K157000(A)</i> | |
- | + | -TEV Protease | |
- | + | -I712078 (C-Terminus) <i>Plate 2, Well 14M, plasmid J70003 (A)</i> | |
- | + | -I712077 (N-Terminus) <i>Plate 2, Well 14K, plasmid J70003 (A)</i> | |
- | + | -pLux | |
- | + | -R0062 (not leaky) <i>Plate 1, Well 6O, plasmid pSB1A2 (A)</i> | |
- | + | -R1062 (median strength in the absence of luxR/HSL) <i>Plate 1, Well 8G, plasmid pSB1A2 (A)</i> | |
- | + | -HO-pcyA | |
- | + | -K098010 <i>Plate 3, Well 11N, plasmid pSB3k3 (K)</i> | |
- | + | -Terminator | |
- | + | -B0015 <i>Plate 1, Well 23L, plasmid pSB1AK3 (AK)</i> | |
- | + | -RFP | |
+ | -E1010 <i>Plate 1, Well 18F, plasmid pSB2K3 (K)</i> | ||
+ | </pre> | ||
+ | </p> | ||
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Last Updated: May 11, 2009 by Fr<sub>3</sub>P</center></font> | Last Updated: May 11, 2009 by Fr<sub>3</sub>P</center></font> |
Revision as of 12:50, 16 July 2009
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Laboratory One: Harry, Bogdan, James, Bryant Laboratory Two: Kate, Mike Tech Support and Laboratory Three: Parthiv, Chris, Chris |
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Laboratory One: Harry, Bogdan, James, Bryant 16/06/2009
-Lab Meeting -Finished the sequence for the surface expression construct -Finalized to-do list -Mini-prepped the following: -Linker K157013 Plate 3, Well 3G, plasmid Bba_K157000(A) -TEV Protease -I712078 (C-Terminus) Plate 2, Well 14M, plasmid J70003 (A) -I712077 (N-Terminus) Plate 2, Well 14K, plasmid J70003 (A) -pLux -R0062 (not leaky) Plate 1, Well 6O, plasmid pSB1A2 (A) -R1062 (median strength in the absence of luxR/HSL) Plate 1, Well 8G, plasmid pSB1A2 (A) -HO-pcyA -K098010 Plate 3, Well 11N, plasmid pSB3k3 (K) -Terminator -B0015 Plate 1, Well 23L, plasmid pSB1AK3 (AK) -RFP -E1010 Plate 1, Well 18F, plasmid pSB2K3 (K) |