Team:Queens/Notebook

From 2009.igem.org

(Difference between revisions)
Line 15: Line 15:
<a href="https://2009.igem.org/Team:Queens"><img src="https://static.igem.org/mediawiki/2009/4/4f/QueensBackground.png"></a>
<a href="https://2009.igem.org/Team:Queens"><img src="https://static.igem.org/mediawiki/2009/4/4f/QueensBackground.png"></a>
-
<br>
+
<br/>
-
<br>
+
<br/>
<div id="nav10d"><a href="https://2009.igem.org/Team:Queens"><img src="https://static.igem.org/mediawiki/2009/2/20/QueensNavHome.png"></a></div>
<div id="nav10d"><a href="https://2009.igem.org/Team:Queens"><img src="https://static.igem.org/mediawiki/2009/2/20/QueensNavHome.png"></a></div>
Line 50: Line 50:
<tr>
<tr>
<td>
<td>
-
<br>
+
<br/>
-
<br>
+
<br/>
</td>
</td>
</tr>
</tr>
Line 58: Line 58:
<td align="left>
<td align="left>
<a name="Lab1"><p style="font-size:150%;font-family:corbel;color:#172C4E;font-weight:bold">Laboratory One: Harry, Bogdan, James, Bryant</p></a>
<a name="Lab1"><p style="font-size:150%;font-family:corbel;color:#172C4E;font-weight:bold">Laboratory One: Harry, Bogdan, James, Bryant</p></a>
-
<br>
+
<br/>
<p style="font-size:120%;font-family:palatino linotype;color:#ECB528">16/06/2009</p>
<p style="font-size:120%;font-family:palatino linotype;color:#ECB528">16/06/2009</p>
<p style="font-size:120%;font-family:palatino linotype;color:#ECB528">
<p style="font-size:120%;font-family:palatino linotype;color:#ECB528">
-
<p>-Lab Meeting
+
<pre>
-
<p>-Finished the sequence for the surface expression construct
+
-Lab Meeting
-
<p>-Finalized to-do list
+
-Finished the sequence for the surface expression construct
-
<p>-Mini-prepped the following:
+
-Finalized to-do list
-
<p>.    -Linker K157013 <i>Plate 3, Well 3G, plasmid Bba_K157000(A)</i>
+
-Mini-prepped the following:
-
<p>.    -TEV Protease
+
-Linker K157013 <i>Plate 3, Well 3G, plasmid Bba_K157000(A)</i>
-
<p>.          -I712078 (C-Terminus) <i>Plate 2, Well 14M, plasmid J70003 (A)</i>
+
-TEV Protease
-
<p>.          -I712077 (N-Terminus) <i>Plate 2, Well 14K, plasmid J70003 (A)</i>
+
-I712078 (C-Terminus) <i>Plate 2, Well 14M, plasmid J70003 (A)</i>
-
<p>.    -pLux
+
-I712077 (N-Terminus) <i>Plate 2, Well 14K, plasmid J70003 (A)</i>
-
<p>.          -R0062 (not leaky) <i>Plate 1, Well 6O, plasmid pSB1A2 (A)</i>
+
-pLux
-
<p>.          -R1062 (median strength in the absence of luxR/HSL) <i>Plate 1, Well 8G, plasmid pSB1A2 (A)</i>
+
-R0062 (not leaky) <i>Plate 1, Well 6O, plasmid pSB1A2 (A)</i>
-
<p>.    -HO-pcyA
+
-R1062 (median strength in the absence of luxR/HSL) <i>Plate 1, Well 8G, plasmid pSB1A2 (A)</i>
-
<p>.          -K098010 <i>Plate 3, Well 11N, plasmid pSB3k3 (K)</i>
+
-HO-pcyA
-
<p>.    -Terminator
+
-K098010 <i>Plate 3, Well 11N, plasmid pSB3k3 (K)</i>
-
<p>.          -B0015 <i>Plate 1, Well 23L, plasmid pSB1AK3 (AK)</i>
+
-Terminator
-
<p>.    -RFP
+
-B0015 <i>Plate 1, Well 23L, plasmid pSB1AK3 (AK)</i>
-
<p>.        -E1010 <i>Plate 1, Well 18F, plasmid pSB2K3 (K)</i>
+
-RFP
 +
-E1010 <i>Plate 1, Well 18F, plasmid pSB2K3 (K)</i>
 +
</pre>
 +
</p>
 +
 
 +
<br/>
 +
 
 +
 
Line 100: Line 107:
<footer>
<footer>
-
<br>
+
<br/>
-
<br>
+
<br/>
-
<br>
+
<br/>
<font color="#172C4E"><center>
<font color="#172C4E"><center>
Last Updated: May 11, 2009 by Fr<sub>3</sub>P</center></font>
Last Updated: May 11, 2009 by Fr<sub>3</sub>P</center></font>

Revision as of 12:50, 16 July 2009



Menu

Laboratory One: Harry, Bogdan, James, Bryant

Laboratory Two: Kate, Mike

Tech Support and Laboratory Three: Parthiv, Chris, Chris



Laboratory One: Harry, Bogdan, James, Bryant


16/06/2009

-Lab Meeting
-Finished the sequence for the surface expression construct
-Finalized to-do list
-Mini-prepped the following:
	-Linker K157013 Plate 3, Well 3G, plasmid Bba_K157000(A)
	-TEV Protease
		-I712078 (C-Terminus) Plate 2, Well 14M, plasmid J70003 (A)
		-I712077 (N-Terminus) Plate 2, Well 14K, plasmid J70003 (A)
	-pLux
		-R0062 (not leaky) Plate 1, Well 6O, plasmid pSB1A2 (A)
		-R1062 (median strength in the absence of luxR/HSL) Plate 1, Well 8G, plasmid pSB1A2 (A)
	-HO-pcyA
		-K098010 Plate 3, Well 11N, plasmid pSB3k3 (K)
	-Terminator
		-B0015 Plate 1, Well 23L, plasmid pSB1AK3 (AK)
	-RFP
		-E1010 Plate 1, Well 18F, plasmid pSB2K3 (K)





Last Updated: May 11, 2009 by Fr3P