Team:Rice/Notebook

From 2009.igem.org

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Eventually this will get formatted differently (better), but until then...
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New practices for this year:
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In order to better preserve our 2009 parts library, whenever you use a new BioBrick, remove the 15uL of water that you resuspended with, transfer it to a PCR tube, and store in the iGEM 2009 box at -20.  Also make sure to list whatever parts you pull on this notebook page.  (And, of course, make glycerol stocks of '''every''' part that you pull from the registry.
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5/23/09
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Thomas
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Pulled parts:
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(Tail switching)
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BBa_I719005 - T7 promoter
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BBa_I719015 - T7 promoter test circuit with GFP
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BBa_B0034 - Strong RBS
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BBa_B0015 - commonly used strong forward terminator
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BBa_B1006 - new, strong forward terminator
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(Tet selection)
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pSB4K5 - low copy Kan vector
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(Part for Dr. Beason)
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BBa_J45200
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from the 2009 library plates.
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Used 1uL of each to HS transform into XL1 competent cells.

Latest revision as of 07:13, 25 May 2009

Eventually this will get formatted differently (better), but until then...

New practices for this year:

In order to better preserve our 2009 parts library, whenever you use a new BioBrick, remove the 15uL of water that you resuspended with, transfer it to a PCR tube, and store in the iGEM 2009 box at -20. Also make sure to list whatever parts you pull on this notebook page. (And, of course, make glycerol stocks of every part that you pull from the registry.

5/23/09

Thomas

Pulled parts:

(Tail switching)

BBa_I719005 - T7 promoter

BBa_I719015 - T7 promoter test circuit with GFP

BBa_B0034 - Strong RBS

BBa_B0015 - commonly used strong forward terminator

BBa_B1006 - new, strong forward terminator

(Tet selection)

pSB4K5 - low copy Kan vector

(Part for Dr. Beason)

BBa_J45200

from the 2009 library plates.


Used 1uL of each to HS transform into XL1 competent cells.