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Project management notebook

Week 1 - BioBricks, primers, protocols and more

Plasmid backbone

We found a plasmid backbone, that works in both e. coli and b. subtilis (that is, both in gram negative and gram positive bacteria).

The plasmid backbone is called BBa_I742123, and can be found here:

It seems that the backbone works with all e. coli type, both we're not a 100%.

The backbone is resistant to the following antibiotics: chloramphenicol, neomycin & gentamicin.

Ordering: we've written HQ, requesting the part since we cant figuar out how to get it via the registry.

Week 2 - ?