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Project task-management notebook


Create biobrick RIP in registry

Create biobrick RIP+Export in registry


Plasmid backbone

Task: Find a backbone for both e.coli and b. subtilis

People: Helle and Mike

July 7th 2009: We found the backbone, BBa_I742123, which other teams have found to be compatible with both both gram positive and gram negative bacteria, such as e. coli.

After talking to HQ, it turns out that the quality of their stock is bad, and they will try to get some home from older teams. Furthermore, it might simply be better to use a more specifik backbone for e. coli and for b. subtilis.


July 8th 2009: We decided to start working with pSB1A3 instead, which is a high-output e. coli backbone.

Output: 100-300 number pr. cell. Resistance: ampicillin (we're going with 50 µg/mL first)


Inducible promoter

Task: Find a promoter, that can be induced

People: Anna

July 7th 2009

I found the inducible promoter BBa_R0011 that is regulated by LacI.

It is a strong promoter, that will be on in stains without LacI.


E. coli strain

Task: Find a e.coli strain


July 7th 2009

The plasmid seems to be working in most types (no need for ccdB) Jacob suggests E. coli KG22, which can turns on the operon by adding IPTG.

July 8th 2009

We'll use Top10 e.coli strain

Promoter for e.coli

Task: Find a constitutive and inducible promoter


July 7th 2009

'Constitutive promoter'

We'll use BBa_J23100 as constitutive promoter. Its located in QC09 Kit Plate 1, Well C18.

'Inducible promoter'

We'll use BBa_R0011 as an inducible promoter. It's located in: QC09 Kit Plate 1, Well 6G, Plasmid: pSB1A2


To induce this promoter region, generally add 1mM of IPTG to a plate (amount will vary by cell strain). That is, total volume of stuff in plate (usually around 0.025 liters) * 0.001 = X amount of liters that you will add to the solution.


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