http://2009.igem.org/wiki/index.php?title=Team:SDU-Denmark/Protocols/Electroporation&feed=atom&action=historyTeam:SDU-Denmark/Protocols/Electroporation - Revision history2024-03-29T09:12:02ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:SDU-Denmark/Protocols/Electroporation&diff=47780&oldid=prevMbarnkob at 10:06, 17 August 20092009-08-17T10:06:06Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Electroporation=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Electroporation=</div></td></tr>
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</table>Mbarnkobhttp://2009.igem.org/wiki/index.php?title=Team:SDU-Denmark/Protocols/Electroporation&diff=28299&oldid=prevMarc.mtk: New page: =Electroporation= This protocol is very useful compared to standard-transformation, because it uses the power of ZAP! Requires less raw material than a normal transformation and is faster...2009-07-27T13:36:32Z<p>New page: =Electroporation= This protocol is very useful compared to standard-transformation, because it uses the power of ZAP! Requires less raw material than a normal transformation and is faster...</p>
<p><b>New page</b></p><div>=Electroporation=<br />
<br />
This protocol is very useful compared to standard-transformation, because it uses the power of ZAP! Requires less raw material than a normal transformation and is faster.<br />
<br />
#Thaw competent cells at room temperature and then keep on ice. 0.2cm cuvettes for electroporation are cooled on ice.<br />
#For each transformation, transfer 40ul cells to a 1.5ml Eppendorf tube and add 1.5ul plasmid from distribution plates. Mix well and transfer to the bottom of a cuvette without making air bubbles. Keep on ice for 5min.<br />
#Place the cuvette in the electroporator and pulse once. Setup: Gene pulser: 25uF 2.5kV Pulse controller: 200ohm<br />
#Immediately add 1ml SOC medium to the cuvette, mix well by pipetting, and transfer to a 1.5ml Eppendorf tube and incubate immediately at 37˚C for 1h on shaker (500 rpm).<br />
#Plate the transformed cells onto LA plates containing antibiotics (100 ug/ml ampicillin). Untransformed cells (negative control) should be plated with and without antibiotics.</div>Marc.mtk