Team:SDU-Denmark/Protocols/Ligations

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(New page: =Ligations= ==Protocol 1== Mix the following: #2uL 10x Ligase buffer #1uL T4 DNA ligase #2 or 4 uL plasmid #5 or 10 uL PCR product Leave at 17 degress C overnight. Use for electropora...)
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Revision as of 11:36, 7 August 2009

Ligations

Protocol 1

Mix the following:

  1. 2uL 10x Ligase buffer
  2. 1uL T4 DNA ligase
  3. 2 or 4 uL plasmid
  4. 5 or 10 uL PCR product

Leave at 17 degress C overnight.

Use for electroporation.

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