"
Team:SDU-Denmark/Protocols/Ligations
From 2009.igem.org
(Difference between revisions)
(New page: =Ligations= ==Protocol 1== Mix the following: #2uL 10x Ligase buffer #1uL T4 DNA ligase #2 or 4 uL plasmid #5 or 10 uL PCR product Leave at 17 degress C overnight. Use for electropora...) |
(→Protocol 1) |
||
| Line 7: | Line 7: | ||
#2uL 10x Ligase buffer | #2uL 10x Ligase buffer | ||
#1uL T4 DNA ligase | #1uL T4 DNA ligase | ||
| - | #2 or 4 uL | + | #2 or 4 uL cut backbone |
| - | #5 or 10 uL PCR product | + | #5 or 10 uL cut PCR product |
Leave at 17 degress C overnight. | Leave at 17 degress C overnight. | ||
Use for electroporation. | Use for electroporation. | ||
Revision as of 11:36, 7 August 2009
Ligations
Protocol 1
Mix the following:
- 2uL 10x Ligase buffer
- 1uL T4 DNA ligase
- 2 or 4 uL cut backbone
- 5 or 10 uL cut PCR product
Leave at 17 degress C overnight.
Use for electroporation.
