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Team:SDU-Denmark/Protocols/Ligations
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Revision as of 11:36, 7 August 2009 by
Marc.mtk
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Ligations
Protocol 1
Mix the following:
2uL 10x Ligase buffer
1uL T4 DNA ligase
2 or 4 uL cut backbone
5 or 10 uL cut PCR product
Leave at 17 degress C overnight.
Use for electroporation.