Team:SDU-Denmark/Protocols/Ligations

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Ligations

Protocol 1

Mix the following:

  1. 2uL 10x Ligase buffer
  2. 1uL T4 DNA ligase
  3. 2 or 4 uL plasmid
  4. 5 or 10 uL PCR product

Leave at 17 degress C overnight.

Use for electroporation.

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