Team:SJTU-BioX-Shanghai

From 2009.igem.org

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This is a template page. READ THESE INSTRUCTIONS.
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
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Welcome to the award winning TU Delft 2008 iGEM wiki! On this page information about the project and its progress can be found. The climax of this project was the Jamboree in Boston, which has taken place at the 8th and 9th of November in 2008. Our team was awarded a golden medal and the trophy for the best wiki.
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== The Team ==
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This is the first year the TU Delft participates in the iGEM competition. We are six undergraduate students, four instructors and a lot of advisors, willing to help out and think with us when this is necessary. An overview of the people involved and our competences can be found on the [[Team:TUDelft/Team|team]] page.
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==The Project - Engineering a biothermometer==
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The goal of our project is to construct temperature-sensing bacteria ''Escherichia coli''  that changes color at different temperatures. Such a thermometer can be applied e.g. as a temperature reporter system in large-scale fermentations, or as a temperature-inducible protein production system. The functionality of this thermometer relies on the post-transcriptional regulation of a temperature-sensitive RNA structure: it opens and enables the ribosome to bind, only when the temperature exceeds a certain threshold.  
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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|align="center"|[[Team:SJTU-BioX-Shanghai | Team Example]]
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[[Image:System.png|thumb|200px|right|Black box representation of the biothermometer, with temperature as input and color as output. The system can be split into two subsystems: A temperature sensitive input system and a color producing output system. The temperature sensitive input system works as a switch, switching on the output system at a certain temperature.]]
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We designed new artificial temperature sensitive RNA sequences, and developed protocols, using luciferase as a reporter, to test their functionality. For the colour output, we built upon the existing carotene biosynthesis pathway and converted all new elements to the BioBrick standard. Furthermore, we developed mathematical models describing both the temperature sensitive parts and the colour mevalonate pathway, and estimated parameters using the experimental data. The ethical issues in design and possible implementation of a commercial product are also addressed.
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!align="center"|[[Team:SJTU-BioX-Shanghai|Home]]
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!align="center"|[[Team:SJTU-BioX-Shanghai/Team|The Team]]
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If you are interested in this project, we invite you to take a closer look at the website. For a more detailed summary of the project, please read the [[Team:TUDelft/Research_Proposal|research proposal]]. For an overview of the results obtained during the summer, please consult the [[Team:TUDelft/Deliverables|deliverables]] section.
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!align="center"|[[Team:SJTU-BioX-Shanghai/Project|The Project]]
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!align="center"|[[Team:SJTU-BioX-Shanghai/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:SJTU-BioX-Shanghai/Modeling|Modeling]]
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!align="center"|[[Team:SJTU-BioX-Shanghai/Notebook|Notebook]]
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Revision as of 10:59, 1 July 2009

[1]

Template:TUDelftiGEM2008 menu home

Welcome to the award winning TU Delft 2008 iGEM wiki! On this page information about the project and its progress can be found. The climax of this project was the Jamboree in Boston, which has taken place at the 8th and 9th of November in 2008. Our team was awarded a golden medal and the trophy for the best wiki.

The Team

This is the first year the TU Delft participates in the iGEM competition. We are six undergraduate students, four instructors and a lot of advisors, willing to help out and think with us when this is necessary. An overview of the people involved and our competences can be found on the team page.

The Project - Engineering a biothermometer

The goal of our project is to construct temperature-sensing bacteria Escherichia coli that changes color at different temperatures. Such a thermometer can be applied e.g. as a temperature reporter system in large-scale fermentations, or as a temperature-inducible protein production system. The functionality of this thermometer relies on the post-transcriptional regulation of a temperature-sensitive RNA structure: it opens and enables the ribosome to bind, only when the temperature exceeds a certain threshold.

Black box representation of the biothermometer, with temperature as input and color as output. The system can be split into two subsystems: A temperature sensitive input system and a color producing output system. The temperature sensitive input system works as a switch, switching on the output system at a certain temperature.

We designed new artificial temperature sensitive RNA sequences, and developed protocols, using luciferase as a reporter, to test their functionality. For the colour output, we built upon the existing carotene biosynthesis pathway and converted all new elements to the BioBrick standard. Furthermore, we developed mathematical models describing both the temperature sensitive parts and the colour mevalonate pathway, and estimated parameters using the experimental data. The ethical issues in design and possible implementation of a commercial product are also addressed.

If you are interested in this project, we invite you to take a closer look at the website. For a more detailed summary of the project, please read the research proposal. For an overview of the results obtained during the summer, please consult the deliverables section.

Template:TUDelftiGEM2008 sidebar