Team:Southampton/Project

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link="#000099" alink="#000099" vlink="#990099"><div id="footerpush"></div>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton">Home</a></li>
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<br>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<br>
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<ul class=" cssMenum">
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<br>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Aims">Project Aims</a></li>
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<br>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Intro">Project Introduction</a></li>
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<br>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
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<br>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Achievements">Project Achievements</a></li>
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<br>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Projection">Project Projection</a></li>
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<!-- http://img195.imageshack.us/img195/8382/a01i.jpg -->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Safety">Project Safety</a></li>
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<!-- http://img233.imageshack.us/img233/928/a01on.jpg --><!-- http://img22.imageshack.us/img22/682/b02rtg.jpg --><!-- http://img218.imageshack.us/img218/5342/b02on.jpg --><!-- http://img200.imageshack.us/img200/1551/c03vvh.jpg --><!-- http://img22.imageshack.us/img22/4571/c03on.jpg --><!-- http://img146.imageshack.us/img146/9978/d04w.jpg --><!-- http://img297.imageshack.us/img297/7195/d04on.jpg --><!-- http://img195.imageshack.us/img195/457/e05a.jpg --><!-- http://img190.imageshack.us/img190/387/e05on.jpg --><!-- http://img200.imageshack.us/img200/650/f06t.jpg --><!-- http://img218.imageshack.us/img218/1472/f06on.jpg --><!-- http://img200.imageshack.us/img200/8735/g07t.jpg --><!-- http://img146.imageshack.us/img146/7450/g07on.jpg --><!-- http://img190.imageshack.us/img190/6283/h08.jpg --><!-- http://img146.imageshack.us/img146/1776/h08on.jpg --><!-- http://img297.imageshack.us/img297/6668/i09.jpg --><!-- http://img297.imageshack.us/img297/9264/i09on.jpg --><!-- http://img22.imageshack.us/img22/5245/j10osz.jpg --><!-- http://img200.imageshack.us/img200/1507/j10on.jpg -->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Refer">References and Links</a></li>
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<div id="B02_"><a
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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  href="https://2009.igem.org/Team:Southampton"
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<ul class=" cssMenum">
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onmouseover="image1.src=loadImage1.src;"
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
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  onmouseout="image1.src=staticImage1.src;"><img
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1">Protocol Theory</a></li>
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name="image1"
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
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  src="http://img22.imageshack.us/img22/682/b02rtg.jpg" border="0"></a></div>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li>
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<div id="C03_"><a
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<li class=" cssMenui"><a class=cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion">DNA Double Digestion</a></li>
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  href="https://2009.igem.org/Team:Southampton/Project"
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel">Gel Electrophoresis</a></li>
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onmouseover="image2.src=loadImage2.src;"
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr">High Fidelity PCR</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/LBplates">LB Plates</a></li>
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style="border: 0px solid ; width: 112px; height: 45px;" alt=""
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation">Ligation</a></li>
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  name="image2"
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
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src="http://img200.imageshack.us/img200/1551/c03vvh.jpg"></a>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
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</div>
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li>
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<div id="D04_"><a
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</ul>
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  href="https://2009.igem.org/Team:Southampton/Parts"
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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style="border: 0px solid ; width: 179px; height: 45px;" alt=""
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<li class=" cssMenui"><a class="  cssMenui" href="#">Biobrick Parts<![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li>
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<div id="E05_"><a
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li>
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  href="https://2009.igem.org/Team:Southampton/Team"
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</ul></li>
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onmouseover="image4.src=loadImage4.src;"
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      <td>
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      <h3>&gt;&gt; Welcome</h3>
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      <p>Southampton University - Project (COMING SOON)</p>
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<div id="maintop"><img style="width: 975px; height: 24px;" id="bg"
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<p>Home | The Team | The Project |
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Biobrick Parts | Modelling |
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<div id="logobox">
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Notebook&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
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<img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /></div>
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University of Southampton <span
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<h1>Project Description</h1>
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  style="font-weight: bold;">iGEM</span>
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2009
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&nbsp;&nbsp;&nbsp; <br>
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  <p>.
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  <p>
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  <blockquote>
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    <p>
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      <br>
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    </p>
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<Br />
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<h4>Rock-Paper-Scissors</h4>
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      <h3>Rock-Paper-Scissors is a
 +
        well-known two-person hand game whereby the players seek to produce a
 +
        gesture that will defeat that of their
 +
        opponent. By coordinating the promoters and operators of three
 +
        different bacterial cells, we have designed a system where each
 +
        bacterium can disable the primary plasmid of specific opponent cells,
 +
        whilst simultaneously enduring the attacks of their rival cells. Our
 +
        aim is to have no bias within the system so that the end result is not
 +
        predetermined.      </h3>
 +
    <h3><a
 +
href="http://en.wikipedia.org/wiki/Rock_paper_scissors">Click here for more information on rock-paper-scissors.</a></h3>
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      <h3>&nbsp;</h3>
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    <p>&nbsp;</p>
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      <table width="64%" border="0" align="center" cellpadding="0" cellspacing="0">
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        <tr bgcolor="#5D5D5D">
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          <td><img src="https://static.igem.org/mediawiki/2009/3/39/Rock.gif" width="417" height="354" border="1"/></td>
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          <td width="0" style="color: #5D5D5D">--</td>
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          <td><object width="441" height="354">
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            <param name="movie" value="http://www.youtube.com/v/6RYTceoRt24&hl=en&fs=1&" />
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            <param name="allowFullScreen" value="true" />
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            <param name="allowscriptaccess" value="always" />
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            <embed src="http://www.youtube.com/v/6RYTceoRt24&hl=en&fs=1&" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="441" height="354"></embed>
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<h3>&nbsp;</h3>
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<h3><br />
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</h3>
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<div id="youtubevideo"></div>
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    <h4>Conway&rsquo;s Game
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      of Life</h4>
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    <h3>John Conway&rsquo;s
 +
      Game of Life is a popular representation of cellular automata. As a
 +
      zero player game, it proceeds without further input once initiated. We
 +
      intend to utilize the Game&rsquo;s basic rules to develop a system
 +
      on two separate plasmids that fluoresces in an imitation of the Game.
 +
      Thereby we aim to initiate and thus control the creation of patterns
 +
      produced by the oscillating bacteria.    </h3>
 +
    <h3><a
 +
href="http://bitstorm.org/gameoflife/">Click here for
 +
      more information on the Game of Life.</a></h3>
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    <p>&nbsp;</p>
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    <table width="48%" border="0" align="center" cellpadding="0" cellspacing="0">
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      <tr>
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        <td><img src="https://static.igem.org/mediawiki/2009/2/21/GoL.gif" width="430" height="132" border="1"/></td>
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    </table>
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    <p>&nbsp;</p>
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    <p>&nbsp;</p>
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    <p>&nbsp;</p>
 +
  </blockquote>
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<a
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href="http://en.wikipedia.org/wiki/Rock_paper_scissors">
 +
C</a></div>
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<div id="sufooter">
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<h5>| <a href="#">Top</a> |&nbsp; &nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp;
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University of
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Southampton iGEM 2009</h5>
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Latest revision as of 19:34, 20 October 2009

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Project Description

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Rock-Paper-Scissors

Rock-Paper-Scissors is a well-known two-person hand game whereby the players seek to produce a gesture that will defeat that of their opponent. By coordinating the promoters and operators of three different bacterial cells, we have designed a system where each bacterium can disable the primary plasmid of specific opponent cells, whilst simultaneously enduring the attacks of their rival cells. Our aim is to have no bias within the system so that the end result is not predetermined.

Click here for more information on rock-paper-scissors.

 

 

--

 


Conway’s Game of Life

John Conway’s Game of Life is a popular representation of cellular automata. As a zero player game, it proceeds without further input once initiated. We intend to utilize the Game’s basic rules to develop a system on two separate plasmids that fluoresces in an imitation of the Game. Thereby we aim to initiate and thus control the creation of patterns produced by the oscillating bacteria.

Click here for more information on the Game of Life.

 

 

 

 

C
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