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Team:Stanford/NotebookPage/22 July 2009
From 2009.igem.org
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* Begin wiki revamp | * Begin wiki revamp | ||
* Learn lab techniques | * Learn lab techniques | ||
| + | *Familiarity w/ protocols for mini-preps and transformations | ||
| + | *Successful mini-prep concentrations | ||
| + | |||
'''Project''' | '''Project''' | ||
| Line 15: | Line 18: | ||
'''Accomplishments''' | '''Accomplishments''' | ||
*Documented Wiki work | *Documented Wiki work | ||
| + | *Two rounds of mini-preps - both had low results | ||
| + | [[Image:MiniPrep2.png |thumb|left|Mini-Prep 2 w/ Epoch Kit ]] | ||
| + | *One Round of transformations | ||
| + | [[Image:T22.png |thumb|left|Transformation Data]] | ||
| + | |||
| + | |||
| Line 21: | Line 30: | ||
'''Problems''' | '''Problems''' | ||
| - | * | + | *Low concentrations/ poor results |
| + | |||
'''Alternative Solutions''' | '''Alternative Solutions''' | ||
| - | * | + | *Get more familiar w/ lab techniques |
| + | *Switch away from kit | ||
| + | |||
| + | '''Protocols''' | ||
| + | *[[Media: Miniprep.doc | Plasmid DNA Miniprep Kit Protocol]] | ||
| + | *[[Media: Transformation.pdf | Transformation protocol | ||
==Events== | ==Events== | ||
Revision as of 06:23, 21 October 2009
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Contents |
Daily Work
Goals
- Write-up and establish Wiki goals
- Begin wiki revamp
- Learn lab techniques
- Familiarity w/ protocols for mini-preps and transformations
- Successful mini-prep concentrations
Project
- Mini-preps and transformations in the lab
Accomplishments
- Documented Wiki work
- Two rounds of mini-preps - both had low results
File:MiniPrep2.png
Mini-Prep 2 w/ Epoch Kit
- One Round of transformations
File:T22.png
Transformation Data
- Made more vBOL images
Problems
- Low concentrations/ poor results
Alternative Solutions
- Get more familiar w/ lab techniques
- Switch away from kit
Protocols
- Plasmid DNA Miniprep Kit Protocol
- [[Media: Transformation.pdf | Transformation protocol
Events
- 10 am wiki meeting with Bobby, Ming, and Joe
- See Accomplishments for Designs
- Goals established and designs made
- 11:30am Mini-preps with Jerome
- Low Yields... will redo in the afternoon
- 2pm Talk Bernard Palsson Clark Center S360
- Systems Biology
- 3:15pm Modeling Team Presentation
- Lab Work w/ Isis
- Transformations
- Mini-preps redo
Comments
Weekly Goals
- Work through the code for templates
