Team:Stanford/NotebookPage/22 July 2009

From 2009.igem.org

(Difference between revisions)
(Daily Work)
(Weekly Goals)
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==Weekly Goals==
==Weekly Goals==
*Work through the code for templates
*Work through the code for templates
 +
*We need to get as many transformations and as much lab work done as possible this week without the genes

Revision as of 06:24, 21 October 2009

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Contents

Daily Work

Goals

  • Write-up and establish Wiki goals
  • Begin wiki revamp
  • Learn lab techniques
  • Familiarity w/ protocols for mini-preps and transformations
  • Successful mini-prep concentrations


Project

  • Mini-preps and transformations in the lab

Accomplishments

  • Documented Wiki work
  • Two rounds of mini-preps - both had low results
File:MiniPrep2.png
Mini-Prep 2 w/ Epoch Kit
  • One Round of transformations
File:T22.png
Transformation Data



  • Made more vBOL images


Problems

  • Low concentrations/ poor results


Alternative Solutions

  • Get more familiar w/ lab techniques
  • Switch away from kit

Protocols

Events

  • 10 am wiki meeting with Bobby, Ming, and Joe
    • See Accomplishments for Designs
    • Goals established and designs made
  • 11:30am Mini-preps with Jerome
    • Low Yields... will redo in the afternoon
  • 2pm Talk Bernard Palsson Clark Center S360
    • Systems Biology
  • 3:15pm Modeling Team Presentation
  • Lab Work w/ Isis
    • Transformations
    • Mini-preps redo

Comments

Weekly Goals

  • Work through the code for templates
  • We need to get as many transformations and as much lab work done as possible this week without the genes