Team:TUDelft/13 July 2009

From 2009.igem.org

(Difference between revisions)
(Weenink)
(Weenink)
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Also did the first transformations to see how it goes:
Also did the first transformations to see how it goes:
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BBa_K145280 GFP-LVA + TetR generators
+
BBa_K145280 GFP-LVA + TetR generators <br>
BBa_K145279 GFP + TetR generators
BBa_K145279 GFP + TetR generators
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after addition of 1 µl rehydrated biobrick DNA, the tubes were incubated for approx 15 (±5) min. on ice. Then one tube of each transformation was heatshocked for 1 min at 42 degrees.
after addition of 1 µl rehydrated biobrick DNA, the tubes were incubated for approx 15 (±5) min. on ice. Then one tube of each transformation was heatshocked for 1 min at 42 degrees.
-
All four transformations were plated out and put at 37 degrees (at 19:00)
+
All four transformations were plated out and put at 37 degrees (at 19:00) <br>
-
BBa_K145280 no heatshock approx 80 microliter on agar+amp plate
+
BBa_K145280 no heatshock approx 80 microliter on agar+amp plate<br>
-
BBa_K145279 no heatshock 50 microliter  on agar+amp plate
+
BBa_K145279 no heatshock 50 microliter  on agar+amp plate<br>
-
BBa_K145280 heatshocked 50 microliter on agar+amp plate
+
BBa_K145280 heatshocked 50 microliter on agar+amp plate<br>
-
BBa_K145279 heatshocked 50 microliter on agar+amp plate
+
BBa_K145279 heatshocked 50 microliter on agar+amp plate<br>
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 21:40, 13 July 2009

13th July


Weenink

prepared 10 agar+amp plates

Also did the first transformations to see how it goes:

BBa_K145280 GFP-LVA + TetR generators
BBa_K145279 GFP + TetR generators

4 vials of DH5alpha comptetent cells were used (2 for each biobrick)

after addition of 1 µl rehydrated biobrick DNA, the tubes were incubated for approx 15 (±5) min. on ice. Then one tube of each transformation was heatshocked for 1 min at 42 degrees.

All four transformations were plated out and put at 37 degrees (at 19:00)
BBa_K145280 no heatshock approx 80 microliter on agar+amp plate
BBa_K145279 no heatshock 50 microliter on agar+amp plate
BBa_K145280 heatshocked 50 microliter on agar+amp plate
BBa_K145279 heatshocked 50 microliter on agar+amp plate