Team:TUDelft/14 September 2009

From 2009.igem.org

Revision as of 23:51, 21 October 2009 by Sriram.tk (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

14 September 2009

Sriram

Today I found that I got 2 different colonies in the plates (LacI generator and Normal RBS Control) left inside desk one very big and clear and others were tiny. I cultured both so that I can do miniprep tomorrow.

Tim Weenink

Did PCR for verificatoin of primer integrity. For this I used last years primers (1 ul of 100mM stock = undiluted), This years primers (1 ul of 100 mM stock = undiluted), 1ul of 25mM of this years primers that were prepared on the 31st of august (= first). 1ul of 25mM of this years primers that were prepared by Sriram and 1ul of 25mM of this years primers that were prepared on the spot.

These reactions were gelled (template DNA was *P, that was shown to give a 1000 bp band before):


Inoculations:

!G2-6 on amp
!H2-6 on amp

!Zg and !Zc on cam and cam&aTc

!G1 in LB, LB&aTc, LB&aTc&amp
!H1 in LB, LB&aTc, LB&aTc&amp