"
Team:TUDelft/15 July 2009
From 2009.igem.org
(Difference between revisions)
(→15th July) |
Tim Weenink (Talk | contribs) (→15th July) |
||
| Line 8: | Line 8: | ||
=='''Weenink'''== | =='''Weenink'''== | ||
The BBa_K145280 had one colony. other ones didnt. It is not reccomendable to use this super fast protocol as it is very unreliable. 30 ml of LB 1x amp. was inoculated with this colony and put in a shaking incubator at 37 degrees and 160 rpm at 10:15. | The BBa_K145280 had one colony. other ones didnt. It is not reccomendable to use this super fast protocol as it is very unreliable. 30 ml of LB 1x amp. was inoculated with this colony and put in a shaking incubator at 37 degrees and 160 rpm at 10:15. | ||
| + | <br> | ||
| + | I transformed DH5a competent cells with B0032, K145015, K145201 biobricks. I used the standard transformation protocol. Cells were incubated for 1h45m. at 37 degrees (accidentally not shaken) in 200µl SOC medium. And 250µl of each transformation mix was plated on amp plates and put in the 37 degrees stove at 16:45. | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 14:53, 15 July 2009
15th July
Sriram
Cool. I got colonies in all the plates. Still more to do...
Weenink
The BBa_K145280 had one colony. other ones didnt. It is not reccomendable to use this super fast protocol as it is very unreliable. 30 ml of LB 1x amp. was inoculated with this colony and put in a shaking incubator at 37 degrees and 160 rpm at 10:15.
I transformed DH5a competent cells with B0032, K145015, K145201 biobricks. I used the standard transformation protocol. Cells were incubated for 1h45m. at 37 degrees (accidentally not shaken) in 200µl SOC medium. And 250µl of each transformation mix was plated on amp plates and put in the 37 degrees stove at 16:45.
