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Team:TUDelft/17 July 2009
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Tim Weenink (Talk | contribs) (New page: {{Template:TUDelftiGEM2009}} ='''17th July'''= =='''Weenink'''== 9:45 inoculated 30 ml LB amp in 37ºC shaking incubator<br> pSB1AK3<br> pSB1AC3<br> pSB1AT3<br> <br><br> 11:30 purified p...) |
Tim Weenink (Talk | contribs) (→Weenink) |
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pSB1AC3<br> | pSB1AC3<br> | ||
pSB1AT3<br> | pSB1AT3<br> | ||
| - | + | <br> | |
11:30 purified plasmids<br> | 11:30 purified plasmids<br> | ||
(in conctrary to protocol i accidentally added >350µl lysis buffer, so i also added 450µl neutralisation buffer) Yield of 100 µl elution:<BR> | (in conctrary to protocol i accidentally added >350µl lysis buffer, so i also added 450µl neutralisation buffer) Yield of 100 µl elution:<BR> | ||
| Line 14: | Line 14: | ||
BBa_K145015: 33.0 ng/µl<br> | BBa_K145015: 33.0 ng/µl<br> | ||
BBa_K145201: 27.8 ng/µl<br> | BBa_K145201: 27.8 ng/µl<br> | ||
| - | + | <br> | |
| + | BBa_K145280 induced with 1mM IPTG at 14:40 | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 14:09, 17 July 2009
17th July
Weenink
9:45 inoculated 30 ml LB amp in 37ºC shaking incubator
pSB1AK3
pSB1AC3
pSB1AT3
11:30 purified plasmids
(in conctrary to protocol i accidentally added >350µl lysis buffer, so i also added 450µl neutralisation buffer) Yield of 100 µl elution:
BBa_B0032: 21.2 ng/µl
BBa_K145015: 33.0 ng/µl
BBa_K145201: 27.8 ng/µl
BBa_K145280 induced with 1mM IPTG at 14:40
