Team:TUDelft/18 August 2009
Lab Notebook
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18 August 2009
Calin
Yesterdays 20 well 1% gel:
Well | Part | Expected Plasmid Size | Status |
1 | |||
2 | |||
3 | |||
4 | |||
5 | |||
6 | DNA Ladder | ✔ | |
7 | Colony PCR 1A - R751ΔtrbK | 1616 | ✖ |
8 | Colony PCR 1A - R751ΔtrbK | 1616 | ✖ |
9 | Colony PCR R751 + pKD46 + trbK_KO_mod_S | 348 | ✔ |
10 | Colony PCR R751ΔoriTR -L-ara +PCR control | ? | |
11 | Colony PCR R751 + pKD46 + oriTR_KO_mod_S | 398 | ✔ |
12 | DNA Ladder | ✔ |
Yesterdays 26 well 2% gel:
Well | Part | Expected Plasmid Size | Status |
1 | DNA Ladder | ✔ | |
2 | |||
3 | |||
4 | |||
5 | |||
6 | |||
7 | |||
8 | |||
9 | |||
10 | |||
11 | |||
12 | |||
13 | |||
14 | |||
15 | |||
16 | |||
17 | |||
18 | |||
19 | DNA Ladder | ✔ | |
20 | |||
21 | |||
22 | Colony PCR 1A - R751ΔtrbK | 1616 | ✖ |
23 | Colony PCR 1A - R751ΔtrbK | 1616 | ✖ |
24 | Colony PCR R751 + pKD46 + trbK_KO_mod_S | 348 | ✔ |
25 | Colony PCR R751ΔoriTR -L-ara +PCR control | ? | |
26 | Colony PCR R751 + pKD46 + oriTR_KO_mod_S | 398 | ✔ |
For the trbK conjugation test 24 plates were made:
Set 1 | |||
Plate ID | Antibiotics | Dilution | |
D1tr | TRI | 100 | |
D2tr | TRI | 10-1 | |
D3tr | TRI | 10-2 | |
D4tr | TRI | 10-3 | |
D5tr | TRI | 10-4 | |
T1tr | TRI + CAM | 100 | |
T2tr | TRI + CAM | 10-1 | |
T3tr | TRI + CAM | 10-2 | |
T4tr | TRI + CAM | 10-3 | |
T5tr | TRI + CAM | 10-4 | |
R1tr | CAM | 100 | |
R2tr | CAM | 10-1 |
Set 2 | |||
Plate ID | Antibiotics | Dilution | |
D6tr | TRI | 100 | |
D7tr | TRI | 10-1 | |
D8tr | TRI | 10-2 | |
D9tr | TRI | 10-3 | |
D10tr | TRI | 10-4 | |
T6tr | TRI + CAM | 100 | |
T7tr | TRI + CAM | 10-1 | |
T8tr | TRI + CAM | 10-2 | |
T9tr | TRI + CAM | 10-3 | |
T10tr | TRI + CAM | 10-4 | |
R3tr | CAM | 100 | |
R4tr | CAM | 10-1 |
For the oriTR conjugation test 51 plates were made:
Set 1 | |||
Plate ID | Antibiotics | Dilution | |
D1ori | TRI + CAM | 100 | |
D2ori | TRI + CAM | 10-1 | |
D3ori | TRI + CAM | 10-2 | |
D4ori | TRI + CAM | 10-3 | |
D5ori | TRI + CAM | 10-4 | |
Ts1ori | AMP + CAM | 100 | |
Ts2ori | AMP + CAM | 10-1 | |
Ts3ori | AMP + CAM | 10-2 | |
Ts4ori | AMP + CAM | 10-3 | |
Ts5ori | AMP + CAM | 10-4 | |
TL1ori | TRI + AMP | 100 | |
TL2ori | TRI + AMP | 10-1 | |
TL3ori | TRI + AMP | 10-2 | |
TL4ori | TRI + AMP | 10-3 | |
TL5ori | TRI + AMP | 10-4 | |
R1ori | AMP | 100 | |
R2ori | AMP | 10-1 |
Set 2 | |||
Plate ID | Antibiotics | Dilution | |
D6ori | TRI + CAM | 100 | |
D7ori | TRI + CAM | 10-1 | |
D8ori | TRI + CAM | 10-2 | |
D9ori | TRI + CAM | 10-3 | |
D10ori | TRI + CAM | 10-4 | |
Ts6ori | AMP + CAM | 100 | |
Ts7ori | AMP + CAM | 10-1 | |
Ts8ori | AMP + CAM | 10-2 | |
Ts9ori | AMP + CAM | 10-3 | |
Ts10ori | AMP + CAM | 10-4 | |
TL6ori | TRI + AMP | 100 | |
TL7ori | TRI + AMP | 10-1 | |
TL8ori | TRI + AMP | 10-2 | |
TL9ori | TRI + AMP | 10-3 | |
TL10ori | TRI + AMP | 10-4 | |
R3ori | AMP | 100 | |
R4ori | AMP | 10-1 |
Set 3 | |||
Plate ID | Antibiotics | Dilution | |
D11ori | TRI + CAM | 100 | |
D12ori | TRI + CAM | 10-1 | |
D13ori | TRI + CAM | 10-2 | |
D14ori | TRI + CAM | 10-3 | |
D15ori | TRI + CAM | 10-4 | |
Ts11ori | AMP + CAM | 100 | |
Ts12ori | AMP + CAM | 10-1 | |
Ts13ori | AMP + CAM | 10-2 | |
Ts14ori | AMP + CAM | 10-3 | |
Ts15ori | AMP + CAM | 10-4 | |
TL11ori | TRI + AMP | 100 | |
TL12ori | TRI + AMP | 10-1 | |
TL13ori | TRI + AMP | 10-2 | |
TL14ori | TRI + AMP | 10-3 | |
TL15ori | TRI + AMP | 10-4 | |
R5ori | AMP | 100 | |
R6ori | AMP | 10-1 |
Tim Weenink
Results of the 24h sequencing are in:
!BPEA1 (R) correct in reliable part of sequencing file !BCEC1 (R) correct in reliable part of sequencing file !CCEA1 (F) !CCEC2 (F) !SPEA1 (F) !SPEA1 (R) !SPEC1 (F) Failed (as expected) !SPEC1 (R) Failed (as expected)
- I6F (F) Perfect
- S3 (F)
- S3 (R)