Team:TUDelft/20 August 2009

From 2009.igem.org

Revision as of 21:42, 20 August 2009 by Tim Weenink (Talk | contribs)

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

20 August 2009

Tim Weenink

We got colonies on yesterdays transformations. RbCl plates had about 20 colonies each, while Electrocompetent cells were hundreds of colonies. Finally an oldfashioned highly efficient transformation. I suspect transformations with larger parts (>1kb) work better.

I inoculated some of the colonies. These are the names of the samples:

R/E stands for RbCl/Electrocompetent (resp) 1C3 stands for the used backbone (pSB1C3) α ß stands for plate numbers 1,2 stands for colony numbers

  • !SIIR1C3α1
  • !SIIR1C3α2
  • !SIIR1C3ß1
  • !SIIR1C3ß2
  • !SIIE1C3α1
  • !SIIE1C3α2
  • !SIIE1C3ß1
  • !SIIE1C3ß2