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Team:TUDelft/21 August 2009
From 2009.igem.org
Lab Notebook
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21 August 2009
Sriram
Today i did miniprep of the biobricks needed for ribo-regulator.
Saeed
!Fr1n downsteam was cut out agarose gel of 19-08-2009 and extracted by gel extraction kit. After extraction DNA concentration was 3.7 ng/microL. !C was digested by SpeI and PstI and !Fr1n downsteam is ligated into this. This construct is called !Z. After ligation !Z was transformed into electrocompetent cells and grown outside the incubator during weekend.
Tim Weenink
Calin
Did colony PCR with Taq on 4 R751_oriTR_KO colonies and 2 R751_trbK_KO colonies.
Mix shotgun sequencing mixes:
CA 1223 bp: A_VF, A_VR
CB 1162 bp: B_VF, B_VR
CC 311 bp: C_VF, C_VR
CE 231 bp: E_VF, E_VR
