Team:TUDelft/27 July 2009

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(Difference between revisions)
(→27 July 2009)
(→Tim Weenink)
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Nanodropped the plasmid isolations I did on friday:<br>
Nanodropped the plasmid isolations I did on friday:<br>
*I is the code for the biobrick I have constructed, that contains the I-SceI cleavage site:<br>
*I is the code for the biobrick I have constructed, that contains the I-SceI cleavage site:<br>
-
*I1:
+
 
-
*I2:
+
{| border="1" align="center"
 +
| Part || DNA concentration in ng/µl || 260/280 || 260/230
 +
|- align="center"
 +
| *I1 || 88.9 || 1.96 || 2.19
 +
|- align="center"
 +
| *I2 || 80.3 || 1.95 || 2.20
 +
|- align="center"
 +
| *I3 || 78.8 || 1.96 || 2.34
 +
|- align="center"
 +
| *I5 || 78.6 || 2.03 || 2.37
 +
|- align="center"
 +
| *I6 || 93.7 || 1.98 || 2.29
 +
|- align="center"
 +
| *I7 || 82.3 || 1.94 || 2.20
 +
|- align="center"
 +
| BBa_K142205 || 73.0 || 1.98 || 2.28
 +
|- align="center"
 +
| !A || 15.3 || 1.97 || 1.67
 +
|- align="center"
 +
|}
 +
 
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 22:28, 27 July 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

27 July 2009

Calin

Placed GFP / RFP plates in fridge. Ordered some trimethoprim. Placed pSB1AK3 cells in tube for culturing. Finished writing Matlab code to plot parameter space. Cleared up confusing equations for mRNA and protein.

Orr

Used mfold to produce 3 locks: one with weak RBS, one with weaker RBS and one with strong RBS.
Using the 2-state hybridization server, combined the key3c with each of the 4 locks (lock3c, weak lock, weaker lock and strong lock) to obtain the hybridised structure.
Created weak key, weaker key and strong key sequences for each of the last three locks by taking the hybridization of each lock with the key3c and correcting the kinks by changing the key3c sequence.
Biobricked the weak, weaker and strong keys into the registry.

Tim Weenink

Nanodropped the plasmid isolations I did on friday:

  • I is the code for the biobrick I have constructed, that contains the I-SceI cleavage site:
Part DNA concentration in ng/µl 260/280 260/230
*I1 88.9 1.96 2.19
*I2 80.3 1.95 2.20
*I3 78.8 1.96 2.34
*I5 78.6 2.03 2.37
*I6 93.7 1.98 2.29
*I7 82.3 1.94 2.20
BBa_K142205 73.0 1.98 2.28
 !A 15.3 1.97 1.67


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