Team:TUDelft/9 September 2009

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(9 September 2009)
 
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===Sriram===
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Today I did the miniprep of the cultures inoculated yesterday. Also did a restriction to analyse the sizes of the products. It can be seen in the gel image below.
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[[Image:Sriram-Delay090909new1.jpg|thumb|center|450px]]
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From the gel it is clear that we have all the assemblies of proper sizes. The only troubling thing was the positive control GFP glowing colony gave a correct band while the non-GFP glowing colony is not of proper size. It is possible due to different assemblies that could be got from electroporation of ligated products.
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Latest revision as of 23:29, 21 October 2009

Lab Notebook

July
MTWTFSS
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6 7 8 9 10 11 12
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20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

9 September 2009

Sriram

Today I did the miniprep of the cultures inoculated yesterday. Also did a restriction to analyse the sizes of the products. It can be seen in the gel image below.

Sriram-Delay090909new1.jpg

From the gel it is clear that we have all the assemblies of proper sizes. The only troubling thing was the positive control GFP glowing colony gave a correct band while the non-GFP glowing colony is not of proper size. It is possible due to different assemblies that could be got from electroporation of ligated products.