Team:TUDelft/Achievements

From 2009.igem.org

(Difference between revisions)
(Time-Delay Device)
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* Successful assembly of parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175023 K175023] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175024 K175024] which correspond to Plasmid 1 and 2 of the Biosynthetic AND gate section respectively.
* Successful assembly of parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175023 K175023] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175024 K175024] which correspond to Plasmid 1 and 2 of the Biosynthetic AND gate section respectively.
* Electroporation of plasmid 1 and 2 into ''Escherichia coli'' TOP10 cells...[[Team:TUDelft/Results2 | more]]
* Electroporation of plasmid 1 and 2 into ''Escherichia coli'' TOP10 cells...[[Team:TUDelft/Results2 | more]]
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'''''Lock and Key Library'''''
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* Construction of an algorithm capable to generate locks (cis regulation) for different ribosome binding sites (RBS’s) and their correspond key (trans).
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* Online lock and key generator derived from the algorithm.
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* Construction of four DNA sequences in biobrick format and pSB1A3 plasmid backbone which encode lock for medium RBS (B0032), key for medium RBS, lock for weak RBS (B0031) or key for weak RBS.
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* Assembly of two composed biobricks, designed to test the functionality of the lock/key pairs constructed.
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* Assembly of two GFP generators under the control of PLacI with weak RBS or medium RBS...[[Team:TUDelft/Results3 | more]]
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Revision as of 13:26, 16 October 2009

The page is still under construction...

Time-Delay Device

Synthetic Transcriptional Cascade

  • Assembly of plasmid 1 and 2 (K175046) and 2 (K175047).
  • Partial confirmation of sequence of both plasmids.
  • Transformation in Escherichia coli
  • Confirmation of both biobrick individual functionality. ... more

Biosynthetic AND gate

  • Successful assembly of parts K175023 and K175024 which correspond to Plasmid 1 and 2 of the Biosynthetic AND gate section respectively.
  • Electroporation of plasmid 1 and 2 into Escherichia coli TOP10 cells... more

Lock and Key Library

  • Construction of an algorithm capable to generate locks (cis regulation) for different ribosome binding sites (RBS’s) and their correspond key (trans).
  • Online lock and key generator derived from the algorithm.
  • Construction of four DNA sequences in biobrick format and pSB1A3 plasmid backbone which encode lock for medium RBS (B0032), key for medium RBS, lock for weak RBS (B0031) or key for weak RBS.
  • Assembly of two composed biobricks, designed to test the functionality of the lock/key pairs constructed.
  • Assembly of two GFP generators under the control of PLacI with weak RBS or medium RBS... more