Team:TUDelft/Achievements

From 2009.igem.org

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* Construction of four DNA sequences in biobrick format and [http://partsregistry.org/wiki/index.php?title=Part:BBa_pSB1A3 pSB1A3] plasmid backbone which encode lock for medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175031 K175031]), key for the lock of medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175032 K175032]), lock for weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175029 K175029]) or key for the lock of weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175030 K175030]).
* Construction of four DNA sequences in biobrick format and [http://partsregistry.org/wiki/index.php?title=Part:BBa_pSB1A3 pSB1A3] plasmid backbone which encode lock for medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175031 K175031]), key for the lock of medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175032 K175032]), lock for weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175029 K175029]) or key for the lock of weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175030 K175030]).
* Assembly of two composed biobricks ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175034 K175034] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175035 K175035]), designed to test the functionality of the lock/key pairs constructed.
* Assembly of two composed biobricks ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175034 K175034] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K175035 K175035]), designed to test the functionality of the lock/key pairs constructed.
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* Assembly of two GFP generators under the control of PLacI with weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175033 K175033]) or medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175048 K175048]).
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* Assembly of two GFP generators under the control of PLacI with weak RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175033 K175033]) or medium RBS ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K175048 K175048])...[[Team:TUDelft/Results3 | more]]
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Revision as of 20:11, 16 October 2009

The page is still under construction...

Time-Delay Device

Synthetic Transcriptional Cascade

  • Assembly of plasmid 1 and 2 (K175046) and 2 (K175047).
  • Partial confirmation of sequence of both plasmids.
  • Transformation in Escherichia coli
  • Confirmation of both biobrick individual functionality. ... more

Biosynthetic AND gate

  • Successful assembly of parts K175023 and K175024 which correspond to Plasmid 1 and 2 of the Biosynthetic AND gate section respectively.
  • Electroporation of plasmid 1 and 2 into Escherichia coli TOP10 cells... more

Lock and Key Library

  • Construction of an algorithm capable to generate locks (cis regulation) for different ribosome binding sites (RBS’s) and their correspond key (trans).
  • Online lock and key generator derived from the algorithm.
  • Construction of four DNA sequences in biobrick format and pSB1A3 plasmid backbone which encode lock for medium RBS (K175031), key for the lock of medium RBS (K175032), lock for weak RBS (K175029) or key for the lock of weak RBS (K175030).
  • Assembly of two composed biobricks (K175034 and K175035), designed to test the functionality of the lock/key pairs constructed.
  • Assembly of two GFP generators under the control of PLacI with weak RBS (K175033) or medium RBS (K175048)... more

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