Team:TUDelft/Achievements
From 2009.igem.org
(Difference between revisions)
Danielsoes (Talk | contribs) |
Danielsoes (Talk | contribs) |
||
Line 4: | Line 4: | ||
=Conjugation System= | =Conjugation System= | ||
- | + | '''''Verification and Characterization of Wild R751''''' | |
*Determination of wild R751 conjugation efficiency. | *Determination of wild R751 conjugation efficiency. | ||
*Design and test of a a method to prevent back propagation of the signal by expressing trbK. This allows having receiver side control over communication. | *Design and test of a a method to prevent back propagation of the signal by expressing trbK. This allows having receiver side control over communication. |
Revision as of 15:08, 20 October 2009
Achievements
Conjugation System
Verification and Characterization of Wild R751
- Determination of wild R751 conjugation efficiency.
- Design and test of a a method to prevent back propagation of the signal by expressing trbK. This allows having receiver side control over communication.
Time-Delay Device
Synthetic Transcriptional Cascade
- Assembly of plasmid 1 and 2 (K175046) and 2 (K175047).
- Partial confirmation of sequence of both plasmids.
- Transformation in Escherichia coli
- Confirmation of both biobrick individual functionality. ... more
Biosynthetic AND gate
- Successful assembly of parts K175023 and K175024 which correspond to Plasmid 1 and 2 of the Biosynthetic AND gate section respectively.
- Electroporation of plasmid 1 and 2 into Escherichia coli TOP10 cells... more
Lock and Key Library
- Construction of an algorithm capable to generate locks (cis regulation) for different ribosome binding sites (RBS’s) and their correspond key (trans).
- Online lock and key generator derived from the algorithm.
- Construction of four DNA sequences in biobrick format and pSB1A3 plasmid backbone which encode lock for medium RBS (K175031), key for the lock of medium RBS (K175032), lock for weak RBS (K175029) or key for the lock of weak RBS (K175030).
- Assembly of two composed biobricks (K175034 and K175035), designed to test the functionality of the lock/key pairs constructed.
- Assembly of two GFP generators under the control of PLacI with weak RBS (K175033) or medium RBS (K175048)... more
Modeling