Team:TUDelft/Achievements

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Achievements

Conjugation System

Time-Delay Device

Synthetic Transcriptional Cascade

  • Assembly of plasmid 1 and 2 (K175046) and 2 (K175047).
  • Partial confirmation of sequence of both plasmids.
  • Transformation in Escherichia coli
  • Confirmation of both biobrick individual functionality. ... more

Biosynthetic AND gate

  • Successful assembly of parts K175023 and K175024 which correspond to Plasmid 1 and 2 of the Biosynthetic AND gate section respectively.
  • Electroporation of plasmid 1 and 2 into Escherichia coli TOP10 cells... more

Lock and Key Library

  • Construction of an algorithm capable to generate locks (cis regulation) for different ribosome binding sites (RBS’s) and their correspond key (trans).
  • Online lock and key generator derived from the algorithm.
  • Construction of four DNA sequences in biobrick format and pSB1A3 plasmid backbone which encode lock for medium RBS (K175031), key for the lock of medium RBS (K175032), lock for weak RBS (K175029) or key for the lock of weak RBS (K175030).
  • Assembly of two composed biobricks (K175034 and K175035), designed to test the functionality of the lock/key pairs constructed.
  • Assembly of two GFP generators under the control of PLacI with weak RBS (K175033) or medium RBS (K175048)... more

Modeling