Team:TUDelft/Project

From 2009.igem.org

(Difference between revisions)
(Project Overview)
(Self-destructive plasmid)
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===Self-destructive plasmid===
===Self-destructive plasmid===
[[Image:TUDelft_logo.png|50px|right|frame]]
[[Image:TUDelft_logo.png|50px|right|frame]]
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Research question:
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'''Research question:'''
Can we achieve cell-to-cell communication:
Can we achieve cell-to-cell communication:
#Using delay device
#Using delay device
#Using self-destructive plasmid
#Using self-destructive plasmid
#Between two F+ cells
#Between two F+ cells
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Goals:
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'''Goals:'''
<br>
<br>
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*Construction and characterization of an inducible or self regulated auto-destructive plasmid
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*Build a delay device that can change the timing of transcribing the target protein
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*Tune the differential expression of (at least) two genes within the same plasmid
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*Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
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*Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid
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*Construct a modified F plasmid that will allow F+/F+ conjugation
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*Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment
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*Clone and incorporation of the plasmid
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<br>
<br>
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Why?
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'''Why?'''
<br>
<br>
*To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
*To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
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*Because it is cool!!!
*Because it is cool!!!
<br><br>
<br><br>
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How?
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'''How?'''
<br>
<br>
Not known yet :D
Not known yet :D
<br><br>
<br><br>
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Applications:
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'''Applications:'''
<br>
<br>
*Cell synchronization
*Cell synchronization

Revision as of 20:18, 3 July 2009

Page is under construction

Project Overview

Our abstract


Finally TUDelft team has a project!!!

Self-destructive plasmid

TUDelft logo.png

Research question: Can we achieve cell-to-cell communication:

  1. Using delay device
  2. Using self-destructive plasmid
  3. Between two F+ cells

Goals:

  • Build a delay device that can change the timing of transcribing the target protein
  • Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
  • Construct a modified F plasmid that will allow F+/F+ conjugation


Why?

  • To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
  • To generate a pulse in the expression of a certain gene
  • To control the amplitude of the pulse created by the plasmid
  • Because it is cool!!!



How?
Not known yet :D

Applications:

  • Cell synchronization
  • Selectable marker recycling
  • Metabolic control analysis
  • Recombination


Project Details


The Experiments


Results