Team:TUDelft/Project

From 2009.igem.org

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''Page is under construction''
 
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='''Project Overview'''=
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== '''Overall project''' ==
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''Our abstract''
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Project Overview is now [[Team:TUDelft | here]]
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<br><br>
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Finally TUDelft team has a project!!!
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<br><br>
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Self-destructive plasmid
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'Goals'
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*Construction and characterization of an inducible or self regulated auto-destructive plasmid
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*Tune the differential expression of (at least) two genes within the same plasmid
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*Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid
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*Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment
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*Clone and incorporation of the plasmid
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Why?
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*To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
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*To generate a pulse in the expression of a certain gene
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*To control the amplitude of the pulse created by the plasmid
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*Because it is cool!!!
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How?
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Not known yet :D
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Applications
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*Cell synchronization
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*Selectable marker recycling
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*Metabolic control analysis
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*Recombination
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*…
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==Project Details==
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==The Experiments==
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==Results==
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Latest revision as of 20:59, 21 October 2009

Project Overview

Project Overview is now here

Retrieved from "http://2009.igem.org/Team:TUDelft/Project"