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Team:TUDelft/Project
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Our abstract | Our abstract | ||
<br><br> | <br><br> | ||
| + | Finally TUDelft team has a project!!! | ||
| + | |||
| + | Self-destructive plasmid | ||
| + | |||
| + | Goals | ||
| + | |||
| + | - Construction and characterization of an inducible or self regulated auto-destructive plasmid | ||
| + | - Tune the differential expression of (at least) two genes within the same plasmid | ||
| + | - Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid | ||
| + | - Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment | ||
| + | - Clone and incorporation of the plasmid | ||
| + | |||
| + | Why? | ||
| + | |||
| + | - To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics | ||
| + | - To generate a pulse in the expression of a certain gene | ||
| + | - To control the amplitude of the pulse created by the plasmid | ||
| + | - Because it is cool!!! | ||
| + | |||
| + | How? | ||
| + | |||
| + | Not known yet :D | ||
| + | |||
| + | Applications | ||
| + | |||
| + | - Cell synchronization | ||
| + | - Selectable marker recycling | ||
| + | - Metabolic control analysis | ||
| + | - Recombination | ||
| + | - … | ||
| + | |||
==Project Details== | ==Project Details== | ||
<br> | <br> | ||
Revision as of 10:52, 29 May 2009
Page is under construction
Overall project
Our abstract
Finally TUDelft team has a project!!!
Self-destructive plasmid
Goals
- Construction and characterization of an inducible or self regulated auto-destructive plasmid - Tune the differential expression of (at least) two genes within the same plasmid - Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid - Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment - Clone and incorporation of the plasmid
Why?
- To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics - To generate a pulse in the expression of a certain gene - To control the amplitude of the pulse created by the plasmid - Because it is cool!!!
How?
Not known yet :D
Applications
- Cell synchronization - Selectable marker recycling - Metabolic control analysis - Recombination - …
Project Details
The Experiments
Results
