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Team:TUDelft/Project
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== '''Overall project''' == | == '''Overall project''' == | ||
| - | Our abstract | + | ''Our abstract'' |
<br><br> | <br><br> | ||
Finally TUDelft team has a project!!! | Finally TUDelft team has a project!!! | ||
| + | <br><br> | ||
Self-destructive plasmid | Self-destructive plasmid | ||
| - | + | <br><br> | |
| - | Goals | + | 'Goals' |
| - | + | <br><br> | |
| - | + | *Construction and characterization of an inducible or self regulated auto-destructive plasmid | |
| - | + | *Tune the differential expression of (at least) two genes within the same plasmid | |
| - | + | *Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid | |
| - | + | *Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment | |
| - | + | *Clone and incorporation of the plasmid | |
| - | + | <br> | |
Why? | Why? | ||
| - | + | <br> | |
| - | + | *To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics | |
| - | + | *To generate a pulse in the expression of a certain gene | |
| - | + | *To control the amplitude of the pulse created by the plasmid | |
| - | + | *Because it is cool!!! | |
| - | + | <br> | |
How? | How? | ||
| - | + | <br> | |
Not known yet :D | Not known yet :D | ||
| - | + | <br> | |
Applications | Applications | ||
| - | + | <br> | |
| - | + | *Cell synchronization | |
| - | + | *Selectable marker recycling | |
| - | + | *Metabolic control analysis | |
| - | + | *Recombination | |
| - | + | *… | |
| + | <br> | ||
==Project Details== | ==Project Details== | ||
Revision as of 10:54, 29 May 2009
Page is under construction
Overall project
Our abstract
Finally TUDelft team has a project!!!
Self-destructive plasmid
'Goals'
- Construction and characterization of an inducible or self regulated auto-destructive plasmid
- Tune the differential expression of (at least) two genes within the same plasmid
- Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid
- Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment
- Clone and incorporation of the plasmid
Why?
- To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
- To generate a pulse in the expression of a certain gene
- To control the amplitude of the pulse created by the plasmid
- Because it is cool!!!
How?
Not known yet :D
Applications
- Cell synchronization
- Selectable marker recycling
- Metabolic control analysis
- Recombination
- …
Project Details
The Experiments
Results
