Team:TUDelft/Project

From 2009.igem.org

(Difference between revisions)
(Bacterial Relay Race)
(Bacterial Relay Race)
Line 20: Line 20:
'''Why?'''
'''Why?'''
<br>
<br>
-
*To send multiple messages to a cell from another induced cell.
+
*To send multiple messages to a cell from an induced cell.
*To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics
*To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics
*To generate a pulse in the expression of a certain gene
*To generate a pulse in the expression of a certain gene
Line 34: Line 34:
<br>
<br>
*Cell synchronization
*Cell synchronization
-
*Multiple bit signals within plasmid DNA comparing to AHL signals
+
*Multiple bit signals within plasmid DNA comparing to single bit AHL signals
*…
*…
<br>
<br>

Revision as of 13:14, 8 September 2009

This page is still under construction

Project Overview

Contents

Our abstract

Bacterial Relay Race


Research question: Can we achieve cell-to-cell communication in bacteria similar to a relay race:

  1. Using delay device
  2. Using self-destructive plasmid
  3. Between two F+ cells

Goals:

  • Build a delay device that can change the timing of transcribing the target protein
  • Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
  • Construct a modified F plasmid that will allow F+/F+ conjugation


Why?

  • To send multiple messages to a cell from an induced cell.
  • To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics
  • To generate a pulse in the expression of a certain gene
  • To control the amplitude of the pulse created by the plasmid
  • Because it is cool!!!


How?
Check out the research proposal page.

Applications:

  • Cell synchronization
  • Multiple bit signals within plasmid DNA comparing to single bit AHL signals


Project Details


The Experiments


Results