Team:TUDelft/Project

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'''How?'''
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Check out the [[Team:TUDelft/Research Proposal|research proposal]] page.
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Check out the indivudual modules' overview page.
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Revision as of 10:35, 7 October 2009

This page is still under construction

Project Overview

Contents

Our abstract

Bacterial Relay Race


Research question: Can we achieve cell-to-cell communication in bacteria similar to a relay race:

  1. Using delay device
  2. Using self-destructive plasmid
  3. Between two F+ cells

Goals:

  • Build a delay device that can change the timing of transcribing the target protein
  • Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
  • Construct a modified F plasmid that will allow F+/F+ conjugation


Why?

  • To send multiple messages to a cell from an induced cell.
  • To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics
  • To generate a pulse in the expression of a certain gene
  • To control the amplitude of the pulse created by the plasmid
  • Because it is cool!!!


How?
Check out the indivudual modules' overview page.

Applications:

  • Cell synchronization
  • Multiple bit signals within plasmid DNA comparing to single bit AHL signals


Project Details


The Experiments


Results