http://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&feed=atom&action=historyTeam:TUDelft/Project - Revision history2024-03-29T09:50:10ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=154985&oldid=prevCplesa: Replacing page with '{{Template:TUDelftiGEM2009}}
='''Project Overview'''=
Project Overview is now here
{{Template:TUDelftiGEM2009_end}}'2009-10-21T20:59:08Z<p>Replacing page with '{{Template:TUDelftiGEM2009}} ='''Project Overview'''= Project Overview is now <a href="/Team:TUDelft" title="Team:TUDelft"> here</a> {{Template:TUDelftiGEM2009_end}}'</p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Project Overview'''=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Project Overview'''=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">===Bacterial Relay Race===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Project <ins class="diffchange diffchange-inline">Overview </ins>is <ins class="diffchange diffchange-inline">now </ins>[[Team:TUDelft <ins class="diffchange diffchange-inline">| here</ins>]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In our project we aim to create a cell-to-cell communication system that allows the propagation of a multi-task message with the capability of being reset. To achieve this, the system will include a reengineered conjugation system, a time-delay genetic circuit and a self-destructive plasmid. This system could be the basis for creating a long distance biosensor and/or be applied in reducing antibiotic resistance of bacteria. </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''</del>Project <del class="diffchange diffchange-inline">description'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Our project </del>is <del class="diffchange diffchange-inline">Bacterial Relay Race. As our team logo and the animation below show we want to build an improved cell to cell communication system. We choose cell to cell communication as subject because of its great importance. Cell to cell communication systems are important because most applications or tasks we set to our synthetic biological systems are generally completed by a population of cells, not a single cell. Gaining new insights at cell to cell communication and designing manageable cell to cell communication systems will provide us a scale of new possibilities. Manageable cell to cell communication systems could have applications in different fields like therapeutic application or fermentation technology applications.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We want to construct an E.coli strain which is capable of passing a GFP signal through conjugation to other E.coli cells only once. Communication appears population wide.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Our work builds on projects of previous iGEM teams from Berkeley UC and Peking University.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''Our project is made up of three modules:'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">1) </del>[<del class="diffchange diffchange-inline">https://2009.igem.org/Team:TUDelft/Conjugation_Overview Conjugation]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We want to build a cell to cell communication system in which communication is based on conjugation. Through conjugation a GFP message is passed to other bacteria in the culture.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">2) </del>[<del class="diffchange diffchange-inline">https://2009.igem.org/</del>Team:TUDelft<del class="diffchange diffchange-inline">/SDP_Overview Self Destructive Plasmid</del>] <del class="diffchange diffchange-inline">(SDP)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The plasmid contains a GFP message, inducible endonuclease gene and restriction site of this endonuclease. The plasmid is able to degrade itself after receiving the destructing signal.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">3) [https://2009.igem.org/Team:TUDelft/Module_3_Overview Delay device]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">It is important that there is a time delay between the moment of passing the SDP and the destructing of it. If the SDP is degraded to fast after conjugation, the degradation of the SDP will not be detected. Therefore we construct a device in this system which delays expression of the endonuclease.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''Some potential applications for this project are'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#Grasping of bacterial communication - the communication between bacteria using DNA can advance through bacterial networks, and in this sense our project would allow to send multiple messages in a single DNA, thereby to better understand those bacterial communication networks.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#Understanding of bacterial antibiotic resistance - these days, bacterial infections are often treated with antibiotics. Unfortunately, bacteria have the tendancy to pass antibiotic resistance to other bacteria through the process of [http://en.wikipedia.org/wiki/Bacterial_conjugation conjugation</del>]<del class="diffchange diffchange-inline">, thus making the antibiotics useless. Using our project, it might be possible for future researchers to better understand the mechanism by which antibiotic resistance passes among bacterial colonies, and perhaps even inhibit it.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#To generate a pulse in the expression of a certain gene</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">#To control the amplitude of the pulse created by the plasmid</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:TUDelftiGEM2009_end}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:TUDelftiGEM2009_end}}</div></td></tr>
</table>Cplesahttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=132629&oldid=prevDanielsoes at 19:48, 20 October 20092009-10-20T19:48:56Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">''This page is still under construction''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Project Overview'''=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Project Overview'''=</div></td></tr>
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</table>Danielsoeshttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=132622&oldid=prevDanielsoes at 19:48, 20 October 20092009-10-20T19:48:41Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Grasping of bacterial communication - the communication between bacteria using DNA can advance through bacterial networks, and in this sense our project would allow to send multiple messages in a single DNA, thereby to better understand those bacterial communication networks.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Grasping of bacterial communication - the communication between bacteria using DNA can advance through bacterial networks, and in this sense our project would allow to send multiple messages in a single DNA, thereby to better understand those bacterial communication networks.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Understanding of bacterial antibiotic resistance - these days, bacterial infections are often treated with antibiotics. Unfortunately, bacteria have the tendancy to pass antibiotic resistance to other bacteria through the process of [http://en.wikipedia.org/wiki/Bacterial_conjugation conjugation], thus making the antibiotics useless. Using our project, it might be possible for future researchers to better understand the mechanism by which antibiotic resistance passes among bacterial colonies, and perhaps even inhibit it</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Understanding of bacterial antibiotic resistance - these days, bacterial infections are often treated with antibiotics. Unfortunately, bacteria have the tendancy to pass antibiotic resistance to other bacteria through the process of [http://en.wikipedia.org/wiki/Bacterial_conjugation conjugation], thus making the antibiotics useless. Using our project, it might be possible for future researchers to better understand the mechanism by which antibiotic resistance passes among bacterial colonies, and perhaps even inhibit it<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#To generate a pulse in the expression of a certain gene</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">#To control the amplitude of the pulse created by the plasmid</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''More applications could be'''</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{Template:TUDelftiGEM2009_end}}</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*To generate a pulse in the expression of a certain gene</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*To control the amplitude of the pulse created by the plasmid</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>Danielsoeshttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=132613&oldid=prevDanielsoes: /* Results */2009-10-20T19:47:52Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*To control the amplitude of the pulse created by the plasmid</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*To control the amplitude of the pulse created by the plasmid</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Results==</del></div></td><td colspan="2"> </td></tr>
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</table>Danielsoeshttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=132608&oldid=prevDanielsoes: /* The Experiments */2009-10-20T19:47:42Z<p><span class="autocomment">The Experiments</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">==The Experiments==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Results==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Results==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:TUDelftiGEM2009_end}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:TUDelftiGEM2009_end}}</div></td></tr>
</table>Danielsoeshttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=132599&oldid=prevDanielsoes: /* Our abstract */2009-10-20T19:47:11Z<p><span class="autocomment">Our abstract</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Project Overview'''=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Project Overview'''=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=='''Our abstract'''==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Bacterial Relay Race===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Bacterial Relay Race===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In our project we aim to create a cell-to-cell communication system that allows the propagation of a multi-task message with the capability of being reset. To achieve this, the system will include a reengineered conjugation system, a time-delay genetic circuit and a self-destructive plasmid. This system could be the basis for creating a long distance biosensor and/or be applied in reducing antibiotic resistance of bacteria. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In our project we aim to create a cell-to-cell communication system that allows the propagation of a multi-task message with the capability of being reset. To achieve this, the system will include a reengineered conjugation system, a time-delay genetic circuit and a self-destructive plasmid. This system could be the basis for creating a long distance biosensor and/or be applied in reducing antibiotic resistance of bacteria. </div></td></tr>
</table>Danielsoeshttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=127377&oldid=prevSriram.tk: /* Bacterial Relay Race */2009-10-20T11:55:09Z<p><span class="autocomment">Bacterial Relay Race</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Understanding of bacterial antibiotic resistance - these days, bacterial infections are often treated with antibiotics. Unfortunately, bacteria have the tendancy to pass antibiotic resistance to other bacteria through the process of [http://en.wikipedia.org/wiki/Bacterial_conjugation conjugation], thus making the antibiotics useless. Using our project, it might be possible for future researchers to better understand the mechanism by which antibiotic resistance passes among bacterial colonies, and perhaps even inhibit it</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Understanding of bacterial antibiotic resistance - these days, bacterial infections are often treated with antibiotics. Unfortunately, bacteria have the tendancy to pass antibiotic resistance to other bacteria through the process of [http://en.wikipedia.org/wiki/Bacterial_conjugation conjugation], thus making the antibiotics useless. Using our project, it might be possible for future researchers to better understand the mechanism by which antibiotic resistance passes among bacterial colonies, and perhaps even inhibit it</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''More applications could be'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*To generate a pulse in the expression of a certain gene</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*To control the amplitude of the pulse created by the plasmid</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==The Experiments==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==The Experiments==</div></td></tr>
</table>Sriram.tkhttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=127370&oldid=prevSriram.tk: /* Project Details */2009-10-20T11:54:08Z<p><span class="autocomment">Project Details</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Understanding of bacterial antibiotic resistance - these days, bacterial infections are often treated with antibiotics. Unfortunately, bacteria have the tendancy to pass antibiotic resistance to other bacteria through the process of [http://en.wikipedia.org/wiki/Bacterial_conjugation conjugation], thus making the antibiotics useless. Using our project, it might be possible for future researchers to better understand the mechanism by which antibiotic resistance passes among bacterial colonies, and perhaps even inhibit it</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Understanding of bacterial antibiotic resistance - these days, bacterial infections are often treated with antibiotics. Unfortunately, bacteria have the tendancy to pass antibiotic resistance to other bacteria through the process of [http://en.wikipedia.org/wiki/Bacterial_conjugation conjugation], thus making the antibiotics useless. Using our project, it might be possible for future researchers to better understand the mechanism by which antibiotic resistance passes among bacterial colonies, and perhaps even inhibit it</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Project Details==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''Research question:'''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Can we achieve cell-to-cell communication in bacteria similar to a relay race:</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#Using delay device</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#Using self-destructive plasmid</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">#Between two F+ cells</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''Goals:'''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Build a delay device that can change the timing of transcribing the target protein</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Construct a modified F plasmid that will allow F+/F+ conjugation</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''Why?'''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*To send multiple messages to a cell from an induced cell.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*To test one condition and return to the original state without killing the cell or changing any of its previous genetic and physiological characteristics</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*To generate a pulse in the expression of a certain gene</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*To control the amplitude of the pulse created by the plasmid</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Because it is cool!!!</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''How?'''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Check out the indivudual modules' overview page.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''Applications:'''</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Cell synchronization</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*Multiple bit signals within plasmid DNA comparing to single bit AHL signals</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*…</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==The Experiments==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==The Experiments==</div></td></tr>
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</table>Sriram.tkhttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=86982&oldid=prevSriram.tk: /* Bacterial Relay Race */2009-10-07T22:28:35Z<p><span class="autocomment">Bacterial Relay Race</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The plasmid contains a GFP message, inducible endonuclease gene and restriction site of this endonuclease. The plasmid is able to degrade itself after receiving the destructing signal.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The plasmid contains a GFP message, inducible endonuclease gene and restriction site of this endonuclease. The plasmid is able to degrade itself after receiving the destructing signal.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>3) [https://2009.igem.org/Team:TUDelft/<del class="diffchange diffchange-inline">Overview </del>Delay device]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>3) [https://2009.igem.org/Team:TUDelft/<ins class="diffchange diffchange-inline">Module_3_Overview </ins>Delay device]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>It is important that there is a time delay between the moment of passing the SDP and the destructing of it. If the SDP is degraded to fast after conjugation, the degradation of the SDP will not be detected. Therefore we construct a device in this system which delays expression of the endonuclease.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>It is important that there is a time delay between the moment of passing the SDP and the destructing of it. If the SDP is degraded to fast after conjugation, the degradation of the SDP will not be detected. Therefore we construct a device in this system which delays expression of the endonuclease.</div></td></tr>
</table>Sriram.tkhttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/Project&diff=86963&oldid=prevSriram.tk at 22:10, 7 October 20092009-10-07T22:10:52Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Project Overview'''=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Project Overview'''=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">__TOC__</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=='''Our abstract'''==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=='''Our abstract'''==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Bacterial Relay Race===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Bacterial Relay Race===</div></td></tr>
</table>Sriram.tk