Team:TUDelft/Project

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Overall project

Our abstract

Finally TUDelft team has a project!!!

Self-destructive plasmid

TUDelft logo.png

Goals:

  • Construction and characterization of an inducible or self regulated auto-destructive plasmid
  • Tune the differential expression of (at least) two genes within the same plasmid
  • Construction of a plasmid which contains the gene of a specific restriction enzyme that, after expression, will destroy the plasmid
  • Construction of a plasmid with enough and specific restriction sites which leads to its destruction under restriction enzyme treatment
  • Clone and incorporation of the plasmid


Why?

  • To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
  • To generate a pulse in the expression of a certain gene
  • To control the amplitude of the pulse created by the plasmid
  • Because it is cool!!!



How?
Not known yet :D

Applications:

  • Cell synchronization
  • Selectable marker recycling
  • Metabolic control analysis
  • Recombination


Project Details


The Experiments


Results