Team:TUDelft/Project

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Project Overview

Contents

Our abstract

Bacterial Relay Race

Research question: Can we achieve cell-to-cell communication in bacteria similar to a relay race:

  1. Using delay device
  2. Using self-destructive plasmid
  3. Between two F+ cells

Goals:

  • Build a delay device that can change the timing of transcribing the target protein
  • Build a plasmid that will produce endonucleases and cut itself into pieces (i.e. destroy itself)
  • Construct a modified F plasmid that will allow F+/F+ conjugation


Why?

  • To test one condition and return to the original state without kill the cell or change any of its previous genetic and physiological characteristics
  • To generate a pulse in the expression of a certain gene
  • To control the amplitude of the pulse created by the plasmid
  • Because it is cool!!!


How?
Check out the research proposal page
Applications:

  • Cell synchronization
  • Multiple bit signals within plasmid DNA comparing to AHL signals


Project Details


The Experiments


Results