http://2009.igem.org/wiki/index.php?title=Team:TUDelft/SDP_Futurework&feed=atom&action=historyTeam:TUDelft/SDP Futurework - Revision history2024-03-28T10:53:51ZRevision history for this page on the wikiMediaWiki 1.16.5http://2009.igem.org/wiki/index.php?title=Team:TUDelft/SDP_Futurework&diff=168020&oldid=prevTim Weenink at 03:30, 22 October 20092009-10-22T03:30:13Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Future Work'''=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>='''Future Work'''=</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The most obvious improvement for future work on a self destructive plasmid would be to replace the LVA-tagged I-SceI homing endonuclease in <partinfo><del class="diffchange diffchange-inline">BBa_175045</del></partinfo> by a wildtype sequence. I-SceI protein with the wildtype sequence is commercially available and has been shown to work. The restriction site and reporter gene have been shown to work, so these would not require immediate attention. Possibly the restriction site could be integrated into the reporter gene sequence, so that cleavage would immediately block transcription of the reporter gene.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The most obvious improvement for future work on a self destructive plasmid would be to replace the LVA-tagged I-SceI homing endonuclease in <partinfo><ins class="diffchange diffchange-inline">BBa_K175045</ins></partinfo> by a wildtype sequence. I-SceI protein with the wildtype sequence is commercially available and has been shown to work. The restriction site and reporter gene have been shown to work, so these would not require immediate attention. Possibly the restriction site could be integrated into the reporter gene sequence, so that cleavage would immediately block transcription of the reporter gene.</div></td></tr>
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</table>Tim Weeninkhttp://2009.igem.org/wiki/index.php?title=Team:TUDelft/SDP_Futurework&diff=167781&oldid=prevTim Weenink: New page: {{Template:TUDelftiGEM2009_menu_M2_SDP}} ='''Future Work'''= The most obvious improvement for future work on a self destructive plasmid would be to replace the LVA-tagged I-SceI homing e...2009-10-22T03:19:50Z<p>New page: {{Template:TUDelftiGEM2009_menu_M2_SDP}} ='''Future Work'''= The most obvious improvement for future work on a self destructive plasmid would be to replace the LVA-tagged I-SceI homing e...</p>
<p><b>New page</b></p><div>{{Template:TUDelftiGEM2009_menu_M2_SDP}}<br />
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='''Future Work'''=<br />
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The most obvious improvement for future work on a self destructive plasmid would be to replace the LVA-tagged I-SceI homing endonuclease in <partinfo>BBa_175045</partinfo> by a wildtype sequence. I-SceI protein with the wildtype sequence is commercially available and has been shown to work. The restriction site and reporter gene have been shown to work, so these would not require immediate attention. Possibly the restriction site could be integrated into the reporter gene sequence, so that cleavage would immediately block transcription of the reporter gene.<br />
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{{Template:TUDelftiGEM2009_end}}</div>Tim Weenink