"
Team:Todai-Tokyo/Notebook/bioclock
From 2009.igem.org
(Difference between revisions)
(→August/September) |
|||
| Line 50: | Line 50: | ||
*cut pUC57 by XhoI and NcoI<BR> | *cut pUC57 by XhoI and NcoI<BR> | ||
→insert GFP, araC or lacI<BR> | →insert GFP, araC or lacI<BR> | ||
| - | * | + | *cut plate1-23L(double terminator) and insert plate1-7L(lacI) in it<BR> |
| + | *PCR of cI~cII<BR> | ||
Revision as of 08:40, 30 September 2009
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
|---|
Contents |
Plan
Aim: Create E.coli that show oscillatory gene expression pattern
Methods:
1. Clone the following genes from Bacillus subtilis into a biobrick vector.
- plate1,13B
- plate1,13L
- plate2,1H
2. Make a gene network that express oscillatory pattern, using these genes.
7/7
Cloning the parts
preculture of the Biobrick parts for Miniprep
7/8
Cloning the parts
Miniprep of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System
failure
7/9
Cloning the parts
Miniprep again of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System
7/29
Miniprep
- P1.14L(araC)
- P1.7L(lacI)
- P1.4E(cI)
- P1.3D(ColE1)
- P1.9C(p15A)
- P1.9G(p15A)
7/30
Miniprep
- P1.14L(araC)
- P1.7L(lacI)
- P3.21D
August/September
- create plasmid including EcoRI restriction site, XbaI restriction site, placI/araC, XhoI restriction site, NcoI restriction site, double terminator, SpeI restriction site and PstI restriction site.→pUC57
-E-X-placI/araC-XhoI-NcoI-double terminator-S-P-
- cut pUC57 by XhoI and NcoI
→insert GFP, araC or lacI
- cut plate1-23L(double terminator) and insert plate1-7L(lacI) in it
- PCR of cI~cII
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Protocols | Ethics |
|---|
