Team:Todai-Tokyo/Notebook/bioclock

From 2009.igem.org

Revision as of 07:48, 9 July 2009 by Tommy (Talk | contribs)

Plan

Aim: Create E.coli that show oscillatory gene expression pattern

Methods:

  1. Clone the following genes from Bacillus subtilis into a biobrick vector.
  1. plate1,7D
  2. plate1,13B
  3. plate1,13L
  4. plate2,1H
  1. Make a gene network that express oscillatory pattern, using these genes.

Cloning the parts

7/7

preculture of following Biobrick parts for Miniprep

7/8

Miniprep of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System

failure

7/9

Miniprep again of E.coli cells containing LDLR gene with Promega, Wizard Plus SV Miniprep DNA Purification System




Home The Team The Project Parts Submitted to the Registry Modeling Notebook
Retrieved from "http://2009.igem.org/Team:Todai-Tokyo/Notebook/bioclock"