Team:Todai-Tokyo/Notebook/bioclock

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Contents

Plan

Aim: Create E.coli that show oscillatory gene expression pattern

Methods:
1. Clone the following genes from Bacillus subtilis into a biobrick vector.


  1. plate1,13B
  2. plate1,13L
  3. plate2,1H

2. Make a gene network that express oscillatory pattern, using these genes.

Cloning the parts

==7/7==

preculture of the Biobrick parts for Miniprep

==7/8==

Miniprep of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System

failure

==7/9==

Miniprep again of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System

7/29

Miniprep

  1. P1.14L(araC)
  2. P1.7L(lacI)
  3. P1.4E(cI)
  4. P1.3D(ColE1)
  5. P1.9C(p15A)
  6. P1.9G(p15A)

7/30

Miniprep

  1. P1.14L(araC)
  2. P1.7L(lacI)
  3. P3.21D


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