Team:Tsinghua/Experiment1

From 2009.igem.org

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(Bottom-Up Approach)
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=Synthesis of the Gene Therapy Production System=
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=Synthesis of the Genome of GenSniper=
==Bottom-Up Approach==
==Bottom-Up Approach==
In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).
In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).

Revision as of 06:38, 3 October 2009

Contents

Synthesis of the Genome of GenSniper

Bottom-Up Approach

In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).

Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET28a and pACYCDuet1.

pET28a pACYCDuet1

Top-Down Approach

GenSniper Production Evaluation