Team:Tsinghua/Experiment1

From 2009.igem.org

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(Bottom-Up Approach)
(Synthesis of the Genome of GenSniper)
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[[Image:pACYCDuet1.jpg|450px|pACYCDuet1]]
[[Image:pACYCDuet1.jpg|450px|pACYCDuet1]]
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[[Image:BUA001.jpg|450px|BUA001]]
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[[Image:BUA001.jpg|800px|center|BUA001]]
==Top-Down Approach==
==Top-Down Approach==
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[[Image:TDA001.jpg|800px|center|TDA001]]
=GenSniper Production Evaluation=
=GenSniper Production Evaluation=

Revision as of 15:46, 9 October 2009

Contents

Synthesis of the Genome of GenSniper

Bottom-Up Approach

In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).

Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET28a and pACYCDuet1.

pET28a pACYCDuet1

BUA001

Top-Down Approach

TDA001

GenSniper Production Evaluation