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From 2009.igem.org

Contents 1 Synthesis of the Gene Therapy Production System 1.1 Bottom-Up Approach 1.2 Top-Down Approach 1.3 Synthesis of the Targeted Biobrick 1.4 Production of the targeted gene therapy vector 1.5 Functioning of the targeted gene therapy vector

Synthesis of the Gene Therapy Production System

Bottom-Up Approach

In the bottom-up approach, we amplify the biobricks from both the bacteriophage lambda and the adenovirus genome and incorporate them with a given order into molecular cloning vector(s).

Specifically, we choose two molecular cloning vectors to encode two sections of the gene therapy vector genome, pET28a and pACYCDuet1.

Top-Down Approach

Synthesis of the Targeted Biobrick

Production of the targeted gene therapy vector

Functioning of the targeted gene therapy vector

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