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Team:Tsinghua/Modeling
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| + | The transcription of λ repressor, notice that λ repressor is coded on the plasmid instead of in the genome of ''E.coli'' | ||
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*[[Image:Eq_4.gif]] | *[[Image:Eq_4.gif]] | ||
| + | The translation of λ repressor | ||
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| - | + | <h3>Plasmid-protein complex affect plasmid replication</h3> | |
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Revision as of 05:47, 4 August 2009
| Home | The Team | The Project | Protocols | Parts | Modeling | Notebook | Brainstorming |
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Contents |
Introduction
Phage production in E.coli
Naked Plasmid Vector Production
One of our team's sub project is the use nakes plasmid binded with a specified protein as the vector of gene therapy DNA. The normal replication process of plasmid will be changed due to the binding of specified protein(in our model,lambda repressor). Considering production of the plasmid protein complex, we want to maxize the amounts of plasmid protein complex, so a balance between the amounts of plasmid and protein is needed. In this model, we want to find out the optimized parameters for this plasmid vector production process.
Reaction kinetics
DNA protein complex’s binding and separating is a dynamical process
The replication and degration of plasmid. The plasmid is considered to replicate in a constant rate (r) without the interference of binding protein. Here the parameter reff means the actual replicating rate of plasmid taken the binding protein into consideration.
The transcription of λ repressor, notice that λ repressor is coded on the plasmid instead of in the genome of E.coli
The translation of λ repressor
Plasmid-protein complex affect plasmid replication
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