Team:Tsinghua/Project

From 2009.igem.org

Revision as of 16:28, 20 August 2009 by Tianf06 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

{| cellpadding="0" cellspacing="0" border="0" width="900" align="center" !align="center"|[[Team:Tsinghua|Home]] !align="center"|[[Team:Tsinghua/Team|The Team]] !align="center"|[[Team:Tsinghua/Project|The Project]] !align="center"|[[Team:Tsinghua/Protocol|Protocols]] !align="center"|[[Team:Tsinghua/Parts|Parts]] !align="center"|[[Team:Tsinghua/Modeling|Modeling]] !align="center"|[[Team:Tsinghua/Notebook|Notebook]] !align="center"|[[Team:Tsinghua/Brainstorming|Brainstorming]] |} {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="70%" align="center" !align="center"|[[Team:Tsinghua/Project Original|Project Original]] !align="center"|[[Team:Tsinghua/Background|Background]] !align="center"|[[Team:Tsinghua/Brainsotrming|Brainsotrming]] !align="center"|[[Team:Tsinghua/Design|Design]] !align="center"|[[Team:Tsinghua/Experiment|Experiment]] !align="center"|[[Team:Tsinghua/Results|Results]] !align="center"|[[Team:Tsinghua/Notebook|Notebook]] |} [[Image:project.png|center|project.png]] Overall Project A relatively significant procedure in gene therapy is to construct a vector to infect target cells and deliver cure gene into them. As a result, the vectors act as big role. And till now researchers use Adeno-associated viruses to do a good job, but the problems of high cost and low production of the virus has not been solved. That is why we attempted to build a highly productive carrier into bacteria. We transformed the structure genes of the phage into bacteria with specific chimera genes attached to the structural genes. We attempted to simulate the Adeno-associated viruses by the phage, for they share the similar structure. Fiber has been attached to the phage to enhance transformation efficiency. Not only we use the cosmid in the phage to carry the cure gene, which has great capability to carry large and multiple genes, but also the cure genes are tissue specific.