Team:Tsinghua/Result3

From 2009.igem.org

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[[Image:C-fiber.png|400px|center|C-fiber]]
[[Image:C-fiber.png|400px|center|C-fiber]]
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=Targeted Biobrick Functioning Characterization=
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=Targeted Biobrick Characterization by Fusing with GFP=
[[Image:transfection.png|600px|center|transfection]]
[[Image:transfection.png|600px|center|transfection]]
[[Image:GFP+Fi.png|600px|center|GFP+Fi-SDSPAGE]]
[[Image:GFP+Fi.png|600px|center|GFP+Fi-SDSPAGE]]

Revision as of 19:23, 18 October 2009

Targeted Biobrick Construction

In this section, we were contructing two contructs in paralell. First, standardized biobrick for parts registry, where Targeted Biobrick can be served for further use by other teams or researchers. Second, incorporate Targeted Biobrick into an expression vector pET-28a to test its expression. Targeted Biobrick was contructed via two rounds of PCR.

Synthesis of Targeted Biobrick. A.pAdEasy-1 plasmid extraction; B. First round PCR to modify fiber gene; C.Second round PCR to modify fiber gene.
pET-28a+Targeted Biobrick
Identification of the Targeted Biobrick positive clones. Desired bands are at 1200-1300 bp
SDS-PAGE of the purified Targeted Biobrick

C-Targeted Biobrick Fusion

C-fiber

Targeted Biobrick Characterization by Fusing with GFP

transfection
GFP+Fi-SDSPAGE
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